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Reference : UMR5798-ALOWUR-003
Workplace : TALENCE
Date of publication : Wednesday, March 18, 2020
Scientific Responsible name : Alois Würger
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 July 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
This theoretical project aims to better understand the movement of proteins along a temperature gradient. Unlike spherical particles and linear DNA, proteins have an irregular shape with non-uniform surface properties (positive and negative charges, hydrophilic and hydrophobic end groups). They also have strong specific ionic interactions with salt (Hofmeister effect) and are sensitive to pH. The “surface” of a protein in a given conformation is made up of the amino acid groups of its constituents, which determine its thermophoretic mobility. Experiments indicate pH and thermoelectric effects, and charge regulation could play a role.
Thermoosmosis and thermophoresis have become standard tools for creating microflows in laser-heated gold nanostructures and self-propelled active particles, as well as for analyzing the binding state of proteins. While the underlying physical mechanisms of colloidal particles and DNA are well understood, the same is not true for protein thermophoresis and its complex dependencies on temperature, salinity and surface properties.
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