Reference : UMR5628-ANNDUC-006
Workplace : GRENOBLE
Date of publication : Wednesday, July 27, 2022
Scientific Responsible name : Charlotte Vendrely (LMGP) & Cédric Picot (CY Cergy Paris Université)
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 November 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
We are looking for a PhD student to work on adhesive proteins inspired from biological glues.
In our daily lives, all conventional man-made adhesives are petrochemical products, which are toxic for the body and show limited efficiency in wet conditions, major drawbacks for biomaterials. In this project, we aim at producing adhesives effective in air and/or in wet conditions inspired by natural glues of some arthropods. This approach using simplified mimics of biological adhesives will facilitate better understanding of systems from which they are derived and could find applications in medicine as well as in biotechnology and industry.
Adhesive proteins of some arthropods have been identified. For these animals, the adhesion mechanism seems to be related to protein self-assembly on the surface. Some molecular basis of this process have been elucidated by our team but its mechanisms remain to be elucidated. Also, we were able to establish a genetic engineering method to produce three protein models inspired by natural adhesive proteins. The structural and self-assembly properties of the recombinant proteins are currently being characterized on various model materials.
The PhD student will pursue this project, in particular, in order to understand the link between the process of self-assembly of proteins and their adhesion to the surfaces of different materials. This project will involve:
-methods of genetic engineering
-production of recombinant proteins
-characterization of proteins in solution and on interfaces using biochemical, biophysical methods and microscopies.
The adhesive properties of the proteins will be investigated.
For applications in medecine, biocompatibility studies via cell biology approaches will also be carried out.
We look for a highly motivated candidate with a strong knowledge in biochemistry, biophysics and/or in material science. The student should be able to work in a team, have very good writing skills (report, presentation…) and good knowledge of at least one of the language used in the lab: French, English.
The Materials and Physical Engineering Laboratory (LMGP) is a joint research unit of the CNRS and Grenoble INP.
It is composed of 3 research teams:
- IMBM (Interaction between Materials and Biological Material),
- FunSurf (functional thin layers and nano-surface engineering),
- NanoMat (Nanomaterials and Advanced Heterostructure)
The laboratory is composed of about 100 people including 40 permanent and about 60 non-permanent (doctoral students, post-doctoral fellows, guest researchers, master students ...).
The LMGP offers a multidisciplinary environment and three research teams. The lab has gained an international reputation in the fields of crystal growth, nanomaterials, structured thin film materials and interactions of proteins with materials. In the lab, the IMBM team is interested in the interactions of proteins with interfaces in particular their adsorption/desorption and their self-assembly by their interactions with materials. Located in the heart of an exceptional scientific environment, the LMGP offers the applicant a rewarding place to work.
The collaborator ERRMECe lab has extensive expertise in the study of different extracellular matrices, on their composition and function, in relationships with cells and the functionalization of materials.
Constraints and risks
Chemical and biological risks
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