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Reference : UMR5535-MICHAH-001
Workplace : MONTPELLIER
Date of publication : Thursday, October 7, 2021
Scientific Responsible name : Michael Hahne
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 January 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Colorectal cancer (CRC) is the third most common and deadly cancer worldwide. CRC is a molecularly highly heterogeneous disease and there is an urgent need to map the heterogeneity of CRC, to identify novel therapeutic intervention points as well as prognostic and predictive biomarkers.
Crucial in the regulation of intestinal homeostasis and pathology is the crosstalk between epithelium lining the crypts and neighbouring fibroblasts. An underexplored trait in cellular crosstalk are primary cilia (PC), an antennae‐like structure extruding the surface of many mammalian cells. PC serve as sensors of environmental signals and regulate a range of signalling pathways associated with carcinogenesis.
We previously reported that colon carcinogenesis, in both mice and human, is associated with a decreased number of PC on colonic fibroblasts (Rocha et al. EMBP J, 2014). The PhD project we offer will explore the biomedical potential of PC and associated pathways in CRC. For this, the student will use tissue specific knock out mice, relevant animal models for CRC, immunohistochemistry, organoid cell cultures, biochemistry and RNAseq. Moreover, we collaborate with the local University Hospital as well as different clinical centers in Europe for the analysis of patient biopsies. Our recent publications illustrate our experimental strategy (Guo et al. J. Clin Invest, 2021; Maurizy et al. bioRxiv 10.1101/2019.12.19.882712, now in press at Nat Commun).
Constraints and risks
You are a highly motivated student with a strong interest in cancer biology and translational biomedical research. Relevant experience with murine models would be advantageous. Fluent proficiency in written and oral communication in English is a prerequisite.
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