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Reference : UMR5306-ISACOM-001
Workplace : VILLEURBANNE
Date of publication : Saturday, September 07, 2019
Scientific Responsible name : Isabelle Compagnon
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2019
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Analysis of lichenic Galactofuranose-containing polysaccharides using IRMPD and ion-mobility: Sugars in their furanose form are not usual in nature but of high interest while analytical tools are limited to recognize them easily, particularly when they are in complex mixtures. So we have created an unprecedented multidisciplinary team-project around the ALGAIMS project to develop innovative and rapid analysis of rare hexofuranosides contained in symbiotic organisms such as lichens. The analytical methods which will be involved have in common the ability to differentiate isomeric sugars according to their conformation. These are infrared multi-photon dissociation spectroscopy (IRMPD, Lyon) and ion mobility mass spectrometry (IM-MS, Nantes). Correlations between both techniques will be established by molecular modeling to better understand the impact of conformational changes on physicochemical properties. Small synthetic oligosaccharides with perfectly defined structures (Rennes) will guarantee reliable analytical results (data banks). Complex matrices containing these units, isolated from lichens (Rennes), will be subjected to specific enzymatic hydrolysis without any impact on the targeted furanosides. Once the method validated, the miniaturization of the detection should allow recognizing the patterns furanose on lichen slices, which is very informative in chemical ecology.
The successful candidate will be recruited at the doctoral school PHAST (Physics ans Astrophysics – Lyon). The work will take place at Institut Lumière Matière in the group « Dynamique Multi-Échelle des Édifices Moléculaires » (group leader: Franck Lépine) under the supervision of Isabelle compagnon. The project is part of the interdisciplinary, ANR funded ALGAIMS project. The consortium brings together specialists of lichen polysaccharide exctraction, and galactofuranoside synthesis (ENSC Rennes) and analytical methods (INRA Nantes).
Constraints and risks
chemical and laser risks. Safety training will be organised by the supervisor
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