Ph.D. in Microfabrication for Biological Imaging and the Cultivation of Biological Cell Assemblies (M/F)

Job Description

Thesis Subject

The PAPILLON project is developing an integrated platform to control and quantify the differentiation of human spheroids derived from induced pluripotent stem cells. The project is structured around three key components: the design and fabrication of the PAPILLON chip, fluidic programming of differentiation, and integration into an automated imaging and analysis system. It is based primarily on the development of a microfluidic chip capable of trapping about ten spheroids under controlled conditions and compatible with advanced imaging during incubation. Within this chip, automated and finely modulated sequences of culture medium perfusion will impose reproducible differentiation trajectories on the spheroids. We will perform real-time imaging of these dynamics to generate mid-throughput data on the physiology of the spheroids. The focus will be on a low-cost and largely open-source approach to lower technological barriers for the community

Your Work Environment

As part of funding from the CNRS Mission for Interdisciplinarity (MITI) for an 80 PRIME project, the doctoral student will work in a highly interdisciplinary environment at the LP2N laboratory and the CRPP. He or she will have access to a wide range of material and human resources in the fields of: 3D cell culture, microfabrication including 3D printing, hydrogels, microfluidics, microscopy, etc. The PhD student will be based at LP2N (CNRS / IOGS / Univ. Bordeaux) in Talence, France, within the BioImaging & Optofluidics (BiOf) team. The BiOf group currently consists of 3 researchers, 2 engineers, 4 postdocs, and 7 PhD students. The BiOf group is highly interdisciplinary (biophysics, optics, biology), and its research topics cover the following areas: - Tissue engineering (using cell capsule technology) - Cellular self-organization (organs-on-a-chip, competition between tumor and healthy tissues, topology of confined epithelia, etc.) - Biophotonics (optical coherence tomography, compact optical devices in an incubator) - Rheology of epithelia and organoids (using in-house developed systems)

Constraints and risks

NA

Compensation and benefits

Compensation

2300 € gross monthly

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

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