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Reference : UMR5248-PATDUL-019
Workplace : PESSAC
Date of publication : Tuesday, June 8, 2021
Scientific Responsible name : Sophie Lecomte
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 September 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The AQUATERS project funded by the ANR aims to understand the impact of amyloid peptides on membranes, for an understanding of their toxicity. The amyloid peptides A1-42 and Tau are involved in the pathology of Alzheimer's disease. These peptides aggregate and interact with the membrane of neuronal cells. At the CBMN Institute, we control the production of peptides and more or less toxic variants. The objective of the thesis is to study the processes of peptide aggregation and their interaction with lipid membranes (for various compositions mimicking the neuronal membrane at different ages of an individual). One of the difficulties is to be able to characterize the different states of peptides, monomers, oligomers and fibers. The strategy is to use methodologies that allow characterization of the morphology of objects but also of their structure at the nanometric scale, the scale of the individual object (AFM, NanoIR and TERS).
The successful candidate will work at the Institute of Chemistry, Biology of Membranes and Nano-objects (CBMN-UMR 5248) in Pessac. Its main activities will be: - The expression and purification of peptides and variants. - Carrying out biochemical and biophysical characterizations of peptides. - The study by ATR-FTIR, AFM and NanoIR of peptide / biomimetic membrane interactions. - Writing a report at the end of the 3 years and thesis defense.
The CBMN Institute is a multidisciplinary research center at the interface of chemistry, biology and physics, comprising about 200 people, half of whom are PhDs, Post-docs and other CDDs. CBMN's research activity revolves around 4 thematic poles and a very rich instrumentation platform at the cutting edge of technology (http://www.cbmn.u-bordeaux.fr/1-presentation). The project will be carried out within 2 research groups of the Multiscale biophysics pole "Spectroscopy and Imaging of peptides active on membranes" under the direction of Dr. Sophie Lecomte (DR CNRS) and "AFM development for biology" under the direction from Prof. Michael Molinari. The laboratory is perfectly equipped for carrying out the experiments.
Constraints and risks
Health and Safety constraints related to the handling of chemicals perfectly supervised within the CBMN institute.
ANR Aquaters funds
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