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Reference : UMR5247-SEBULR-002
Workplace : MONTPELLIER
Date of publication : Thursday, July 22, 2021
Scientific Responsible name : Sebastien ULRICH
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Overview of the project. The SACSI project will study the self-assembly of unprecedented pH-responsive amphiphilic dynamic covalent polymers for generating targeted nano-vectors of siRNA. The ambition of this project is to discover self-fabricated multi-component siRNA vectors that adapt throughout the different steps of the delivery process in order to maximize efficacy. We have recently reported a 1st targeted dynamic covalent polymer which is formed in situ by siRNA templating (Angew. Chem. Int. Ed., 2021, 60, 11, 5783-5787). We now wish to exploit this proof-of-concept to identify effective vectors that are selected by live cells, essentially performing dynamic combinatorial chemistry on cells.
Context and mission. The PhD student will be in charge of bioorganic synthesis of modified peptides and lipid conjugates (SPPS, HPLC chromatography), including pH-responsive aromatic tweezers (Nanoscale, 2017, 9, 31-36), in vitro biochemical analyses (gel electrophoresis, DLS, Zéta potential) and cell studies (MTT and functional assays in 96-well plates format, using UV-Vis read-out).
Ph.D. candidate. You have recently graduated (M.Sc., Ecole d'Ingénieur, Master II, pharmacy) with excellent theoretical and practical knowledge in bioorganic chemistry (synthesis, purification, characterization). You have a strong interest for the interface with biology, and possibly for drug and gene delivery in particular, with a desire to learn and practice. We expect solid know-how on chemical synthesis to kick start the project – appropriate training will then be provided for the subsequent tasks at the chemistry-biology interface. You are a self-starter, working autonomously, willing to take initiatives and eager to work in a multidisciplinary project. You show a clear enthusiasm for research. You are a team player with very good communication skills. Knowledge of French would be useful but is not essential. Starting date: October 2021 (flexible), salary: ca. 1700 €/month.
Host laboratory. We belong to the Institut des Biomolécules Max Mousseron (IBMM) and will be located, from September 2021, in a brand-new building on the CNRS campus in Montpellier, France. The institute is part of the “Pole Chimie Balard” excellence cluster in chemistry which is supported by the I-SITE MUSE (Montpellier University of excellence).
Constraints and risks
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