Description du sujet de thèse

Building on our recent work demonstrating the stabilization of nucleic acid-based architectures through the formation of boronate esters (DNAzymes and ribozymes), this PhD project aims to develop and validate a new method for the direct selection of functional aptamers from libraries of 5'-boronic acid-modified oligonucleotides. This approach will integrate stabilization criteria directly into the selection process, offering a route to highly stable and functional split aptamer systems without the need for prior isolation of the full-length aptamer.
The PhD student will synthesize a library of phosphoramidite building blocks with a 5'-boronic acid function. These modified building blocks will be used to prepare oligonucleotide sequences and evaluated using a selection protocol targeting various molecules of interest. The selection experiments will be carried out in collaboration with Prof. Sabine Müller's group at the University of Greifswald (Germany).

The candidate (M/F) must have :
- good knowledge of organic chemistry
- a strong interest in nucleic acids
- communication and writing skills (French and English)
- The candidate must be familiar with environmental, health and safety (EHS) requirements.

Contexte de travail

Institut des Biomolécules Max Mousseron
Equipe ChemBioNAC
www.chembionac.com

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.