Description of the thesis topic

Building on our recent work demonstrating the stabilization of nucleic acid-based architectures through the formation of boronate esters (DNAzymes and ribozymes), this PhD project aims to develop and validate a new method for the direct selection of functional aptamers from libraries of 5'-boronic acid-modified oligonucleotides. This approach will integrate stabilization criteria directly into the selection process, offering a route to highly stable and functional split aptamer systems without the need for prior isolation of the full-length aptamer.
The PhD student will synthesize a library of phosphoramidite building blocks with a 5'-boronic acid function. These modified building blocks will be used to prepare oligonucleotide sequences and evaluated using a selection protocol targeting various molecules of interest. The selection experiments will be carried out in collaboration with Prof. Sabine Müller's group at the University of Greifswald (Germany).

The candidate (M/F) must have :
- good knowledge of organic chemistry
- a strong interest in nucleic acids
- communication and writing skills (French and English)
- The candidate must be familiar with environmental, health and safety (EHS) requirements.

Work Context

Institut des Biomolécules Max Mousseron
Equipe ChemBioNAC
www.chembionac.com

The position is located in a sector under the protection of scientific and technical potential (PPST), and therefore requires, in accordance with the regulations, that your arrival is authorized by the competent authority of the MESR.