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Reference : UMR5235-KARKIS-002
Workplace : MONTPELLIER
Date of publication : Wednesday, July 29, 2020
Scientific Responsible name : Karima KISSA & Andrea PARMEGGIANI
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 2 November 2020
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
This project is a continuation of the biophysical characterization and modeling of the emergence of blood stem cells in the zebrafish embryo.
Funding for a 36-month doctoral thesis has been granted by the CNRS as part of its Prime 80 program and is available from 1 November 2020 at the University of Montpellier, France.
The zebrafish embryo, thanks to its transparency and the recent development of molecular tools and transgenic fluorescent reporter lines, is a relevant in vivo model to study the different steps leading to definitive haematopoiesis. Imaging of live zebrafish embryos during the first days of development showed that multipotent precursors (HSCs) emerged directly from the floor of the aorta through a stereotypic process involving a strong flexion preceding the exit of single endothelial cells from the aortic ventral wall and their concomitant transformation into hematopoietic cells. We have named this process the endothelial hematopoietic transition (EHT) (Kissa & Herbomel, Nature 2010; Poullet at al, CMLS 2019).
The aim of the project is to elucidate the molecular and biomechanical events leading to the emergence of hematopoietic stem cells in the zebrafish embryo. Previous studies suggested that shear stress resulting from arterial pressure was important for the emergence of HSC. This project will focus on the potential relationships between pulsatile flow, mechanical deformation of the aorta and the EHT process as a consequence of aortic constriction. The project will be carried out among the teams of Karima KISSA (Emergence of hematopoietic strain cells and cancer, UMR5235), Andrea PARMEGGIANI (Biological Physics and Systems Biology, UMR 5235 and Complex Systems & Nonlinear Phenomena, UMR5221) and Sergei ROCHAL (South Federal University of Rostov on Don, Russia). The aim of the latter will be to design mathematical models of the EHT process in correlation with the experimental data.
The UMR5235 and UMR5221 laboratories are very stimulating environments for working in the fields of biology and biophysics.
We are looking for a highly motivated candidate with a background in theoretical biophysics and biomechanics with knowledge of cell biology. Autonomy, organization and communication are essential skills to work with our research teams.
The selected candidate will be required to register at the University of Montpellier.
The successful candidate will carry out research under the supervision of Drs Karima KISSA (LPHI-UMR5235) and Andrea PARMEGGIANI (L2C- UMR5221) in Montpellier and Sergei ROCHAL (South Federal University of Rostov on Don, Russia). The co-supervisors will have regular meetings with the PhD student in order to ensure the follow-up of the research and training. In addition, an Individual Monitoring Committee will be set up by the Scientific Experts to monitor the progress of the doctoral student's work and the progress of the thesis.
Constraints and risks
Travel in France and abroad is likely.
Applicants should provide a single PDF document including 1) a cover letter, 2) a detailed CV specifying the final grades of M1 and M2 separately, 3) a one-page summary of their scientific project carried out in the Master's program, and 4) the names and e-mail addresses of two people who can recommend the applicant.
The deadline for sending applications is 15/09/2020. Successful candidates will be interviewed by videoconference between 20/09 and 30/09/2020 and the starting date from 01/11/2020.
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