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Reference : UMR5168-GIOFIN-003
Workplace : GRENOBLE
Date of publication : Thursday, April 01, 2021
Scientific Responsible name : Giovanni Finazzi
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Phytoplankton are highly diverse and play a key role in food webs, O2 production and CO2 sequestration. The question of responses of planktonic microalgae to changing environments is becoming increasingly relevant. Global warming is making oceans are becoming warmer and more oligotrophic, limiting photosynthesis. While satellite and omics analysis has illustrated the consequences of climate change on phytoplankton, our understanding of physiological responses at the single cell level remains poor. This thesis project aims to understand the structural and metabolic responses of microalgae to environmental change.
Microalgae will be grown under different conditions (e.g. different nutrient sources, temperatures and light levels) and their responses will be characterised by single cell imaging of chlorophyll fluorescence. The ecophysiological responses will be complemented by high definition imaging of subcellular changes, with a particular focus on chloroplasts, using FIB-SEM (Focused Ion Beam Scanning Electron Microscopy). The novel combination of advanced structural and structural and chemical imaging will provide a detailed scenario of acclimatisation to light and nutrient stress in phytoplankton at the cellular and subcellular levels.
The person recruited will join the "Light, Photosynthesis and Metabolism" team (http://lpm-research.com/), which is made up of about fifteen people and is interested in the regulation of the photosynthesis mechanism through a multidisciplinary approach. The selected candidate will work in close collaboration with the project leader (Giovanni Finazzi) and the PhD co-supervisor Florence Courtois, MdC UGA
The host team is part of the Cellular and Plant Physiology Laboratory (LPCV), which is a joint CNRS-University of Grenoble Alpes-CEA-INRA research unit composed of about one hundred collaborators, located at the CEA on the Scientific Polygon in Grenoble
The ERC "ChloroMito", aims to characterize the role of subcellular energy interactions in optimizing photosynthesis in marine phytoplankton.
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