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Reference : UMR5086-CHRGRA-003
Workplace : LYON 07
Date of publication : Monday, December 28, 2020
Scientific Responsible name : Christophe Grangeasse
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 3 May 2021
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
The pneumococcus is in the WHO list of priority pathogens for research and development of new antibiotics. Recently, we have determined qualitatively and quantitatively the changes in protein phosphorylation occurring upon the induction of competence, a genetic differentiation program which allows the acquisition of new genetic traits and that is recognized as the leading force of bacterial adaptation to stresses such as antibiotic treatments. A main goal will be to study the most interesting candidates, including proteins of unknown function, for their role in the pneumococcal physiology and the impact of their phosphorylation. Importantly, we will evaluate the contribution of the candidate to cell division and/or DNA transformation and their potential function in connecting the two processes. In fundamental terms, it will allow to establish new mechanisms of regulation in a major bacterial model organism. In applied terms, this could lead to innovative working hypotheses to combat bacterial antibiotic resistance. For this work, we will employ an integrative approach combining genetics, proteomics, protein biochemistry and cell imaging. All these approaches are routinely used in the lab.
The candidate will be part of the team "Pathogenic Bacteria and Phosphorylation of Proteins" of the MMSB unit. He/she will contribute to the team's main research topic concerning the pneumococcal cell cycle. The team currently consists of 6 permanent staff (DR, CR and MC) and 5 non-permanent staff (M2, doctoral students, post-docs).
He / she will work on a daily basis in relation with the researchers and students of the team. The project is part of a collaborative program with European funding from the Marie Sklodowska-Curie Actions - Innovative Training Network. The candidate will therefore have to meet and discuss with the other doctoral students of the program. He/she will also be required to do short-term assignments (2 months maximum) on secondment to project partners in Germany and Italy.
Constraints and risks
The candidate will work in Level 2 Security Lab and in a Radio-Protected Environment.
Applicants must not have resided or carried out their main activity (work, studies, etc.) in France for more than 12 months in the 3 years immediately prior to the date of appointment.
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