Description du sujet de thèse

The PhD student will work on optimising a super-resolution microscope by locating single molecules at cryogenic temperature.

Activities :
- Optimising optical instrumentation for super-resolution fluorescence microscopy at cryogenic temperature
- Functionalise the surface of microscope slides for in vitro studies
- Perform cell culture for in cellulo studies
- Collect the data obtained
- Optimise data processing
- Take an interest in the photophysics of fluorescent proteins
- Present results at group meetings/seminars and international conferences

Skills :
- Expertise in optical instrumentation for microscopy
- Programming expertise (Matlab, Python)
- Knowledge of fluorescent proteins
- Notions of cell biology, biochemistry and molecular biology
- Scientific rigour and organisational skills
- Ability to work independently and as part of a team in a multidisciplinary environment.
- English (written/oral) at least level C1 of the European Framework of Reference for Languages

Contexte de travail

Located on the Grenoble Science Park (ESRF/ILL/EMBL/IBS), the Institut de Biologie Structurale (IBS) is a major national and international player in the field of integrated structural biology. As a research centre, technical platform, host site and scientific training centre, the IBS is dedicated to developing research in structural biology, a key area of research for understanding fundamental biological mechanisms. It is supported by 10 state-of-the-art platforms.
As a joint research unit (CEA-CNRS-UGA), the IBS is made up of 20 research groups, each offering a multi-disciplinary approach at the frontiers of biology, physics and chemistry, in line with 3 research areas. Nearly 320 people work there, including researchers, doctoral students, engineers and technicians, in a multicultural and international environment.
The new recruit will join the Pixel team, which works on fluorescent proteins and the development of super-resolution microscopy (https://www.ibs.fr/fr/recherche/assemblage-dynamique-et-reactivite/groupe-imagerie-integree-de-la-reponse-au-stress/equipe-pixel/).

Contraintes et risques

Laser risks, cryogenic risks, chemical and biological risks.

Research will be carried out in either French or English, although non-French-speaking candidates are expected to make every effort to learn basic French in order to facilitate communication and integration into the laboratory.