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Thesis on the structural and functional study of enzymes targeting complex polysaccharides (M/F)

This offer is available in the following languages:
- Français-- Anglais

Date Limite Candidature : jeudi 15 juin 2023

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Informations générales

Intitulé de l'offre : Thesis on the structural and functional study of enzymes targeting complex polysaccharides (M/F) (H/F)
Référence : UMR5075-CYNDAV-041
Nombre de Postes : 1
Lieu de travail : GRENOBLE
Date de publication : jeudi 13 avril 2023
Type de contrat : CDD Doctorant/Contrat doctoral
Durée du contrat : 36 mois
Date de début de la thèse : 1 octobre 2023
Quotité de travail : Temps complet
Rémunération : 2 135,00 € gross monthly
Section(s) CN : Molecular and structural biology, biochemistry

Description du sujet de thèse

The thesis project aims at studying enzymes involved in the depolymerization and desulfation of complex polysaccharides of the glycosaminoglycan family.

Activities :
- Expression and purification of enzymes in recombinant form (expression in eukaryotic systems)
- Functional assays (in vitro enzyme assays, or cellular assays)
- Biophysical characterization (MALLS, MP, SPR, BLI....)
- Structural study by X-ray crystallography.

Contexte de travail

Located on the Grenoble Science Park (ESRF/ILL/EMBL/IBS), the Institute of Structural Biology (IBS) is a national and international player in the field of integrated structural biology. The IBS is a research center, a technical platform, a host site and a scientific training center. Its mission is to develop research in structural biology, a field of research that is crucial to the understanding of fundamental biological mechanisms. It is supported by 12 state-of-the-art platforms.
As a joint research unit (CEA-CNRS-UGA), the IBS is composed of 19 research groups, each of which proposes a multi-disciplinary approach, at the frontiers of biology, physics and chemistry, in coherence with 3 research axes. Nearly 300 people work there as researchers, PhD students, engineers and technicians in a multicultural and international environment.
The person recruited will join the SAGAG-Vivès team, which works on Glycosaminoglycan polysaccharides, their interactions with proteins, and the enzyme mechanisms regulating their structure and function. The team consists of a research director, two engineers and a PhD student."

Contraintes et risques

The research will be carried out in either French or English, however it is expected that the non-French speaking candidate will strive to learn the basics of French in order to facilitate communication and integration into the laboratory.