Reference : UMR5075-BRUFRA1-002
Workplace : GRENOBLE
Date of publication : Thursday, March 31, 2022
Scientific Responsible name : Bruno Franzetti
Type of Contract : PhD Student contract / Thesis offer
Contract Period : 36 months
Start date of the thesis : 1 October 2022
Proportion of work : Full time
Remuneration : 2 135,00 € gross monthly
Description of the thesis topic
Mission: The mission takes place within the framework of a research program financed by the Auvergne-Rhône Alpes region (Pack Ambition Research Nutri-CLEC project). The objective of the thesis, the financing of which has already been secured, is to enable the use of extremophile enzymes in the agro-food biotechnology sector by developing their immobilization in the form of crystals. This technology is based on the unique structural and biochemical properties of proteolytic enzymes isolated from abyssal organisms discovered at the Institute of Structural Biology in partnership with IFREMER as well as on a new generation of nucleating agents developed by the Company. Versatile. In a context of environmental and economic crisis, these immobilized enzymes are intended to produce biostimulants for food from various sources of natural biomass.
Activities: The research program will include the development of crystallization protocols, the characterization of the enzymatic and biophysical properties of the immobilized enzymes and the study of their efficiency in the treatment of biomass. At the same time, we will seek to better understand the mode of 'action' of these enzymes in order to optimize their efficiency by mutagenesis and to develop the engineering of multi-catalytic enzymatic complexes.
Education: The candidate must have a background in biochemistry with an interest in structural enzymology and agro-food biotechnologies. Training in chemistry with a biochemistry and/or biotechnology component may also be considered.
The Institute of Structural Biology is a research unit affiliated with the University of Grenoble-Alpes, the CNRS and the CEA. The institute currently employs 280 people, including many students and post-docs from different countries. It has high-performance and internationally competitive facilities in molecular and cellular biology, biochemistry, biophysics and structural biology. The Institute is a founding member of the Partnership for Structural Biology with prestigious pan-European institutes: the European source of synchrotron radiation, the European center for neutron scattering, the Grenoble station of EMBL, all located nearby and offering an environment strong international. In this context, the ELMA group (website: http://www.ibs.fr/recherche/groupes-de-recherche/groupe-extremophiles-et-grands-assemblages-moleculaires-b-franzetti/) specializes in structural biochemistry of archaic cellular machinery with a focus on intracellular proteolysis. The laboratory develops specific tools in crystallography and small-angle scattering for the study of large assemblies. The proposed subject is part of a collaboration with IFREMER in Brest. Supervision will be provided by 2 DR-level researchers. The skills present in the ELMA group and access to the panel of biophysical characterization platforms of the IBS will allow the candidate to acquire a solid training in structural biochemistry/nanotechnologies.
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