Reference : UPR9022-BENSTE-026
Nombre de Postes : 1
Workplace : STRASBOURG
Date of publication : Monday, January 23, 2023
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 April 2023
Proportion of work : Full time
Remuneration : From 2833 € /month gross/month based on the experience
Desired level of education : PhD
Experience required : Indifferent
Our goal is to increase our knowledge of virus-vector interactions though the identification of host and viral genes and pathways that control the process and outcomes of arbovirus infections in Aedes aegypti mosquitoes. We will use a combination of cutting-edge “omics” (genomics and transcriptomics) approaches, molecular biology, and transgenesis techniques to identify and characterize the mechanisms of mosquito resistance to arboviruses and the molecular basis of arbovirus infection in mosquitoes. This information could provide new possibilities and tools to design efficient control strategies to interrupt vectorial transmission.
Grow arboviruses and perform infections in mosquitoes and cell culture
Performa RNA silencing in mosquitoes and cell lines
Generate transgenic and or CRISPR/Cas9 mosquitoes
Analyse gene expression by quantitative RT-PCR, immunoblot.
Genome-wide transcriptome/genome analysis
Prepare samples for mass spectrometry and analyse results
Prepare manuscripts for publication in international journals.
Prior knowledge and experience in virology, molecular biology, immunology, entomology and/or bioinformatics
Prior experience working with insects and insect genetics
Knowledge in molecular biology techniques (cloning, PCR, qPCR, CRISPR/Cas9 system)
Design, execution and analysis of transcriptomics and/or genomics
English level C1 according to the Common European Framework of Reference for Languages; C1 corresponding to the level of autonomy
Capacity to write manuscripts
Capacity to read and analyse the relevant literature in the field
Autonomy, ability to critically analyse own work and that of colleagues with constructive suggestions
Ability to coach trainees and students
The selected applicant will join a young Atip-avenir team at the Research Unit M3i 'Insect Models of Innate Immunity' in the Institut de Biologie Moléculaire et Cellulaire on the central campus of the University of Strasbourg. The M3i unit focuses on the cellular and molecular bases of the potent immune system of insects, using not only Aedes mosquitoes but also the fruit fly Drosophila melanogaster and Anopheles mosquitoes as models. The applicant will also greatly benefit from the dynamic and the multidisciplinary interaction with the other units hosted in the institute both scientifically and technically. This project will be co-advised by Dr. João Marques (Research director at CNRS UPR9022, Strasbourg, France) and Dr. Fabien Aubry (CNRS researcher and Atip-avenir fellow at UPR9022, Strasbourg, France).
Constraints and risks
Usual constraints related to laboratory work in biology and follow-up of experiments and maintenance of mosquito lines.
Work in P3 confined laboratory for experiments with dengue and Zika viruses.
Mosquito duties on Saturday-Sundays (3h once every 3-4 months).
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