Reference : UPR9022-BENSTE-023
Nombre de Postes : 1
Workplace : STRASBOURG
Date of publication : Wednesday, November 30, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 February 2023
Proportion of work : Full time
Remuneration : From 2833 € euros gross/month based on the experience
Desired level of education : PhD
Experience required : Indifferent
The mission is to to study the STING pathway in the model organism Drosophila melanogaster. STING is a critical molecule of the antiviral innate immune pathway in mammals, whose activation or deregulation is involved in several types of pathologies beyond infectious diseases (e.g., cancer, neurodegenerative diseases). In Drosophila, STING participates in antiviral innate immunity and is activated by cyclic dinucleotides produced by at least two cGAS-like receptors (cGLR), which sense the presence of viruses. The research project will exploit the assets of the Drosophila model to decipher the in vivo functions of cGLRs and STING, from nucleic acid sensing to activation of transcription, with the aim of shedding light on some still poorly understood aspects of cGAS/STING biology in mammals.
Drosophila genetics: maintenance and crosses of drosophila strains, epistasis analysis, production of mutant or transgenic lines
Analysis of gene expression by quantitative RT-PCR, immunoblot
Biochemical analysis of the composition of the molecular complexes
Cell biology: maintaining cell lines, genetically modifying them and studying their properties in culture
Determination of the intracellular localisation of proteins in the course of viral infection and CDNs injection
Production and titration of viral stocks
Knowledge on fly genetics and of its physiology, of host defences or molecular virology
English level C1 according to the Common European Framework of Reference for Languages; C1 corresponding to the autonomous level
Autonomy, ability to criticise/analyse his-her personal work and that of colleagues with constructive proposals
Ability to coach trainees and students
Diploma required: PhD in molecular and cellular biology/immunology/virology or genetics
The selected candidate will join a multinational team entitled "Antiviral Reaction-Effector Mechanisms" within the laboratory “Insect Models of Innate Immunity (M3i)”, one of three laboratories hosted by the Institute of Molecular and Cellular Biology (IBMC) located on the central campus of the University of Strasbourg, France. This unit is dedicated to the study of molecular and cellular bases of anti-infectious defense, using as a model the fruit fly drosophila (Drosophila melanogaster and more than 10 other drosophila species). This team is directed by Professor Jean-Luc Imler and is composed of 10 people.
Constraints and risks
Usual constraints related to laboratory work in biology and follow-up of experiments and maintenance of drosophila strains.
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