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Reference : UPR9022-BENSTE-005
Workplace : STRASBOURG
Date of publication : Monday, November 04, 2019
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 January 2020
Proportion of work : Full time
Remuneration : From 2643 euros gross/month based on the experience
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
The mission is to conduct research on insect model organisms to understand the link between mitochondrial respiration and antiviral immunity. More specifically, research will be conducted on the fly model Drosophila melanogaster to characterize the role of subunits from the complex I of the mitochondrial respiratory chain in antiviral defences. The results obtained will be tested in Aedes aegypti mosquitoes in the context of infections with human viruses such as dengue or Zika.
Maintenance of drosophila strains and genetic crosses, production of mutant or transgenic lines
Analysis of gene expression by quantitative RT-PCR, immunoblot
Determination of the intracellular localisation of proteins by immunofluorescence
Biochemical analysis of the composition of the molecular complexes of the respiratory chain
Monitoring the metabolic activity of mitochondria
Maintaining cell lines, genetically modifying them and studying their properties in culture
Production and titration of viral stocks
Knowledge on fly genetics and of its physiology, of host defences or molecular virology
Expert in English language
Autonomy, ability to criticise/analyse his-her personal work and that of colleagues with constructive proposals
Ability to coach trainees and students
Diploma required: PhD in molecular and cellular biology/immunology/virology or genetics
The selected candidate will join a multinational team entitled "Antiviral Reaction-Effector Mechanisms" within the laboratory “Insect Models of Innate Immunity”, one of three laboratories hosted by the Institute of Molecular and Cellular Biology located on the central campus of the University of Strasbourg, France. This unit is dedicated to the study of molecular and cellular bases of anti-infectious defense, using as a model the fruit fly drosophila (Drosophila melanogaster) or mosquitoes.
The project is the subject of an international collaboration with the laboratories of Pr. Joao T. Marques (Department of Biochemistry and Immunology at Universidade Federal de Minas Gerais, Brazil), who will co-supervise the work, and Dr. Arnaud Mourier (BioDynaMit group leader at IBGC UMR5095-CNRS / Bordeaux University, France).
Constraints and risks
Usual constraints related to laboratory work in biology and follow-up of experiments and maintenance of drosophilic strains.
Work in P3 confined laboratory for experiments with dengue and Zika viruses.
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