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Reference : UPR9002-SEBPFE-002
Workplace : STRASBOURG
Date of publication : Monday, November 15, 2021
Type of Contract : FTC Scientist
Contract Period : 36 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : 2690 to 3821 € gross salary depending on prior experience
Desired level of education : PhD
Experience required : Indifferent
The project will consist in studying the innate immune response during infection of human cells, mosquitoes and ticks by the Zika virus (ZIKV) and tick-borne encephalitis virus (TBEV). More specifically, the aim will be to characterize the interactome of the viral RNA replication intermediate in order to identify new factors in antiviral response. Mass spectrometry and high throughput sequencing approaches will be implemented in these infection models.
The main activities will be to:
-establish cellular models and infection conditions for ZIKV and TBEV
-carry on the experiments to identify proteins interacting with viral RNAs by mass spectrometry
-validate and functionally characterize the identified candidate proteins
-determine their role during viral infection using high-throughput approaches
-The scientist will have an excellent command of biochemistry, molecular and cellular biology techniques used in the study of host-virus interactions
-She/he will have some experience in virology, if possible in the handling of level 3 pathgenic viruses
-She/he will have some knowledge in the analysis of proteomics and/or transcriptomics datasets
-She/he will be able to handle the project autonomously and communicate effectively in english (B2 level).
The scientist will be recruited in the frame of an ANR project performed in collaboration between two laboratories based at the Institut de Biologie Moléculaire et Cellulaire (IBMC) in Strasbourg, France. She/he will be working in the research unit "Architecture and Reactivity of RNA", which comprises 10 groups working on different aspects of RNA biology and infectious diseases. She/he will work under the supervision of Sébastien Pfeffer, CNRS research director, head of the team "non coding RNAs and viral infections". In addition, she/he will work in the research unit "Insect models of innate immunity", which comprises 7 groups working on immune response in insects. She/he will work under the supervision of Carine Meignin, Professor at the Strasbourg University and head of the team "RNA interference and receptors". The research scientist will benefit of a stimulating scientific environment and will have full access to all equipments and facilites of both units and institute that are necessary for the completion of the project.
Constraints and risks
Handling of level III pathogenic viruses means that it will be necessary to work in BSL3 laboratories where strict health and safety rules are implemented.
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