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Reference : UPR9002-MICRYC-007
Workplace : STRASBOURG
Date of publication : Thursday, October 14, 2021
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 13 December 2021
Proportion of work : Full time
Remuneration : between 2700 and 3800 € gross monthly depending on experience
Desired level of education : PhD
Experience required : Indifferent
The droplet microfluidic technology used by the "Digital RNA Biology" team (Institute of Molecular and Cellular Biology, Strasbourg) allows the production and the manipulation (e.g., controlled fusion and sorting) of water-in-oil microdroplets of a few picoliters, very homogeneous in size, and at a rate of several thousand events per second. These droplets are usually functionally inert microreactors, i.e. not interfering with the encapsulated molecules or biological reactions, and allow the realization of various screening and analyses at ultrahigh-throughput regimes. These compartments are also characterized by an important surface/volume ratio, a property that remains largely unexploited. To overcome this lack, a collaboration with Alain WAGNER's team (Faculty of Pharmacy, Illkirch) has allowed the development of a technology for functionalizing the internal surface of droplets by grafting specific capture biomolecules (oligonucleotides, antibodies) onto the surfactant, an amphiphilic molecule that stabilizes the droplet by placing itself at the water/oil interface.
The present project will aim to use biomolecule capture technology to decorate the inner surface of droplets with synthetic RNA motifs. Gene mutant libraries of RNA-binding proteins will then be generated and each gene will be expressed as a fusion protein coupled to a fluorescent reporter (e.g., GFP) within the functionalized droplets. Thus, protein mutants able to bind to the immobilized RNA motif will be easily identified by the relocalization of protein fluorescence on the surface of the droplets. It will thus be possible to identify protein mutants capable of recognizing predefined RNA motifs and to generate new specific artificial RNA/protein pairs. This approach will also allow the identification of molecules capable of interfering with the RNA/protein interaction.
The person recruited will be in charge of:
- setting up and validating the technology for the specific capture of RNA motifs on the surface of droplets.
- prepare and validate prototypes of RNA binding proteins fused with a fluorescent protein
- to produce and validate mutant libraries of RNA binding protein genes
- implement and perform the selection of reprogrammed protein mutants with new specificity
- analyze, interpret and compile the results, and write a manuscript
- present the collected data in scientific meetings and international congresses
- Have a Ph.D. in molecular biology or chemobiology from January 1, 2017 onwards.
- Possess a demonstrated expertise in molecular biology and ideally have experience in RNA manipulation and protein production.
- To be able to work efficiently, independently, and as part of a team
- Have an attraction for multidisciplinary projects
The research work will be carried out in the team "Digital Biology of RNA" headed by Michael RYCKELYNCK and hosted in the CNRS unit "Architecture and Reactivity of RNA" (Institute of Molecular and Cellular Biology, Strasbourg). The team has a unique and internationally recognized expertise in the development and use of droplet microfluidics for the study of RNA, including the development of fluorogenic RNA aptamers of biotechnological interest. The team is currently composed of two professors-researchers, two post-doctoral researchers, three PhD students and two engineers.
The work will take place in the framework of a collaborative research project financed by the University of Strasbourg and will involve a close collaboration with the team of Alain WAGNER (Faculty of Pharmacy, Illkirch).
The post-doctoral fellow will have access to all the equipment and infrastructure necessary for the successful completion of the project. On the other hand, in addition to an informal daily follow-up of the work, weekly meetings will be organized with the scientific leader as well as exchanges with the team will take place within the framework of the weekly laboratory meetings.
For more information on the team and its activities, please visit the website:
Constraints and risks
No particular constraint or risk.
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