General information
Offer title : Post-doctoral researcher M/F in molecular microbiology (H/F)
Reference : UPR9002-ISACAL-002
Number of position : 1
Workplace : STRASBOURG
Date of publication : 30 September 2024
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 3 February 2025
Proportion of work : Full time
Remuneration : between 3021,50€ and 3451,51€
Desired level of education : Niveau 8 - (Doctorat)
Experience required : 1 to 4 years
Section(s) CN : Molecular and structural biology, biochemistry
Missions
The aim of the project will be to analyse the function of regulatory RNAs and of a ribonuclease (RNase) from Staphylococcus aureus in biofilms and/or in an infectious context. In particular, the mission will be to determine the modulation of the expression of this RNase as well as its RNA targets in bacteria in biofilms or infecting immune cells. Various high-throughput sequencing approaches will be used to analyse RNA expression under these different conditions.
Activities
Activities will involve (1) setting up transcriptome and RIP-seq experiments and (2) implementing models of infection of immune cells by different strains of Staphylococcus aureus.
Skills
- The researcher should have knowledge and skills in the physiology of pathogenic bacteria and RNA biology.
- The researcher may have experience in cell culture.
- The researcher should be able to manage the project independently.
- The researcher should have a good command of written and spoken English (B2 level).
Work Context
The researcher will be assigned to the 'RNA Architecture and Reactivity' unit, which comprises 11 teams working on various aspects of RNA biology in relation to various infectious pathologies in humans. He/she will work in the 'RNA Regulation in Pathogenic Bacteria' team, renowned for its work on the study of translation and its control in bacteria, and on the role of RNA in the regulation of gene expression, particularly in Staphylococcus aureus. He/she will work under the supervision of Isabelle Caldelari, associate professor at the University of Strasbourg, as part of an ANR project, the main aim of which is to study the functions of RNase J2 and regulatory RNAs in lifestyle decisions in Bacillus subtilis and Staphylococcus aureus. The researcher will benefit from a stimulating scientific environment and will have access to the equipment and technical facilities of the unit and the institute required for the project.
Constraints and risks
Handling class II pathogenic bacteria will require working in LSB2 laboratories where hygiene and safety rules will have to be applied.