Faites connaître cette offre !
Reference : UPR5301-ISACAL-081
Workplace : GRENOBLE
Date of publication : Tuesday, November 16, 2021
Type of Contract : FTC Scientist
Contract Period : 13 months
Expected date of employment : 1 February 2022
Proportion of work : Full time
Remuneration : between 2663 and 3783 € gross monthly depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
The fight against antibiotic resistance, a major public health issue, requires a detailed analysis of the metabolism of the bacterial wall and more particularly of its major component: peptidoglycan. This polymer, composed of saccharide chains cross-linked by peptide stems, is particularly complex and difficult to access for biological studies. Within the framework of an ANR project in collaboration with the Institute of Structural Biology (IBS Grenoble), we are looking for a post-doctoral fellow who will develop the chemo-enzymatic synthesis of peptidoglycan oligosaccharides for the structural and functional study of proteins involved in the biosynthesis of the bacterial wall.
The person recruited will have the mission to synthesize and characterize a set of complex oligosaccharides modified or not by peptide stems. To do so, he/she will carry out chemical and enzymatic reactions, purify (by chromatography on silica, ion exchange or steric exclusion...) and characterize the molecules produced by mass spectrometry and NMR. He/she will evolve in a multidisciplinary environment at the interface of organic chemistry, biochemistry and synthetic biology.
The candidate should have a PhD in chemistry with solid experience in organic synthesis. Skills in glycochemistry will be appreciated. He or she should be highly motivated for research, well organized and autonomous.
This project will take place in the Chemistry and Biotechnology of Oligosaccharides CBO team of CERMAV (UPR CNRS 5301). CBO is a multidisciplinary team at the interface of glycochemistry, biochemistry and microbiology whose research activities are focused on the production of oligosaccharides and glycoconjugates for structure-function studies in the fields of human health and plants. For this purpose, CBO develops chemo-enzymatic approaches based on the use of glycoside hydrolases (Marine Drugs 2021), glycosynthases (Chem. Eur. J. 2021) or glycosyltransferases through the metabolic engineering of bacteria (Biomolecules 2020).
We talk about it on Twitter!