Missions

Red pulp macrophages (RPMs) in the spleen ensure the continuous phagocytosis of aged or damaged red blood cells. We are studying how the metabolism of RPMs adapts to this function, particularly the contribution of autophagy. To do this, we are using a transgenic mouse line with a deletion of a key autophagy gene specifically in macrophages. This work is helping us to better understand the reduction in autophagic flux observed in age-related diseases and its impact on cellular metabolism and tissue macrophage homeostasis.

Activities

Isolation and characterization of primary cells from mouse biological samples (skin, spleen, lymph nodes)
Gene expression analysis by quantitative RT-PCR.
Flow cytometry
Immunohistochemistry on frozen and paraffin sections
Prepare manuscripts for publication in international journals.

Skills

Prior knowledge and experience in molecular biology, preparation of primary cells from mouse organs, immunology.
Level 1 animal experimentation training certificate (designer)
Knowledge of molecular biology techniques (PCR, qPCR)
English level B1 according to the Common European Framework of Reference for Languages; B1 corresponding to the level of autonomy
Ability to write manuscripts
Ability to read and analyze relevant literature in the field.
Autonomy, ability to critically analyze one's own work and that of colleagues by making constructive suggestions.
Ability to supervise interns and students

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Work Context

The person recruited will join the CNRS UPR3572/I2CT laboratory at the Institute of Molecular and Cellular Biology (IBMC) located on the central campus of the University of Strasbourg, France.
• A major player in fundamental research worldwide, the French National Center for Scientific Research (CNRS) is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to combine different scientific disciplines to shed light on and understand the challenges of the contemporary world, in collaboration with public and socio-economic actors.• The CNRS UPR3572/I2CT “Immunology, Immunopathology and Therapeutic Chemistry” laboratory focuses on the molecular and cellular foundations of the immune response and its dysfunctions with the aim of defining new therapeutic avenues for targeted immuno-intervention in the treatment of autoimmune, tumor and viral diseases. It has state-of-the-art equipment in cytometry, microscopy, and transcriptomics. • The “Interactions of the Immune System with its Microenvironment” group studies the functional relevance of interactions between innate and adaptive immune cells and their immediate environment (mesenchymal cells, endothelial cells, epithelial cells, and neurons) in health and disease. The proposed work, led by V Flacher (CNRS Research Director), is based on collaboration with partners in the ANR AUTOMATE consortium (EL Gautier, ICAN, Paris; F Gros, CRBS, Strasbourg).

Constraints and risks

Usual constraints associated with cell biology laboratory work.
Usual constraints associated with animal experimentation.
Work in a BSL2 confined laboratory.

Additional Information

Working hours: 38.5 hours per week, Monday to Friday, daytime hours.
The Institute is located in the heart of the University of Strasbourg's central campus, accessible by bus and tram. The CNRS contributes to the cost of private health insurance and public transportation. An administrative restaurant is available on campus. Meals are covered by the CNRS.