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Reference : UPR3212-DAVHIC-007
Workplace : STRASBOURG
Date of publication : Monday, February 03, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 15 March 2020
Proportion of work : Full time
Remuneration : 44 176 euros annual salary before deductions (3 766€ monthly before deductions)
Desired level of education : PhD
Experience required : 1 to 4 years
Ensure daily monitoring of diurnal rodents used for the project; Manage the experimental protocols; Perform visual non-invasive analyses of treated and control animals every 2 months; Take post-mortem eye tissue samples; Undertake structural and functional analyses of vision in diurnal rodents.
- ensure the welfare of the different groups of animals throughout the project;
- install the lighting devices, make sure they work properly;
- perform non-invasive visual exams every 2-3 months for the different groups;
- carry out post-mortem sampling on subgroups of animals every 2-3 months;
- perform cellular and molecular analyses: histology, immunochemistry, molecular biology (qPCR), melatonin assay, membrane lipid characterization;
- meticulously archive the experimental data in a laboratory notebook
- candidate with a good knowledge of neuroscience, preferably of the visual system;
- candidate with a good experience of handling small animals (rodents), knowledge of anesthesia protocols for small animals;
- candidate with theoretical and practical knowledge of electroretinography;
- candidate with theoretical and practical knowledge of current cell and molecular biology techniques: quantitative histology, immunohistochemistry, western blotting, extraction and purification of RNAs and proteins, qPCR, lipid analysis;
- careful, serious, autonomous person able to work in a team.
The project will be carried out at the INCI CNRS UPR3212 (https://inci.u-strasbg.fr/) on the campus of the University of Strasbourg, and more specifically within the team "Light, vision and brain" https://inci.u-strasbg.fr/?page_id=435. The team currently has> 15 people, including 7 permanent researchers, 2 post-docs and 7 thesis students. Current projects concern the characterization of the retinal circadian clock, the molecular mechanisms involved in hereditary and environmental retinal degenerations, the neural pathways involved in the effects of light on behavior, and the metabolism of cholesterol in the pathophysiology of retina. The team is part of a group of laboratories whose scientific interests converge on the role of the circadian clock in health.
Constraints and risks
No particular constraints
The project is funded by ANSES (French National Agency for Food Safety, Environment and Work) to better understand the effects of overnight exposure to artificial light, specifically the possible effects on visual health.
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