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Reference : UPR2357-PASGEN-004
Workplace : STRASBOURG
Date of publication : Wednesday, September 15, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2021
Proportion of work : Full time
Remuneration : from € 2,675.27 gross monthly
Desired level of education : PhD
Experience required : 1 to 4 years
The post-doctoral fellow will be part of the" Cellular functions of ubiquitin-dependent proteolysis " and will be in charge of the study of post-translational regulations under stress conditions of the Arabidopsis AGO1 protein. The candidate will purify the AGO1 protein from Arabidopsis thaliana plants under conditions of normal growth and abiotic stress. The study will include half-life analyzes of proteins, sequencing of small RNAs associated with AGO1, phenotyping of mutants in the “RNA silencing” pathway and confocal microscopy.
- Functional study and crossing of Arabidopsis mutants in the "RNA silencing" pathway
- Purification of AGO1, its protein factors and associated small RNAs
- NGS analyses
- Analysis of the subcellular location of AGO1 under stressful conditions
The post-doctoral fellow must have a good training in plant molecular biology, biochemistry or cell biology. Other essential criteria for this position are strong motivation, autonomy, thinking and problem-solving skills, excellent communication skills in English and a strong work ethic. Previous experience in RNA biology and plant genetics will be a plus. Fluency in reading, writing and speaking English, level B2 according to the Common European Framework of Reference for Languages will be required.
The research work will be carried out at the Institute of Molecular Biology of Plants (IBMP) in Strasbourg. The candidate will have the opportunity to interact with two other teams involved in the project within the same unit.
Constraints and risks
Usual constraints and risks of a plant molecular biology project. No external mobility is planned.
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