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Reference : UMR9198-MURGON-002
Workplace : SACLAY
Date of publication : Thursday, June 10, 2021
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 September 2021
Proportion of work : Full time
Remuneration : Monthly gross salary between €2,728 and €3,881 depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
The candidate is recruited in the framework of a scientific project of the national challenge "Biomedicine" with the theme of new expression systems. His/her mission will be to develop and optimize the bioproduction of therapeutic proteins by cell-free systems.
He/she will be in charge of setting up and optimizing a cell-free system for the efficient and low-cost production of antimicrobial peptides containing non-canonical amino acids. This transcription and translation system will be reconstituted from recombinant elements essentially from the bacterium Escherichia coli. The incorporation of non-canonical amino acids into the target peptides will be achieved by a genetic code reprogramming method together with flexizyme-acylated tRNAs. The candidate will also set up a high-throughput functional assay to evaluate the antimicrobial activity of the peptides produced.
- expertise in protein expression in Escherichia coli, purification of peptides/proteins by various chromatographic methods, protein characterization by various biochemical and biophysical methods (in particular mass spectrometry)
- strong knowledge in molecular biology; experience in the manipulation of RNA molecules and their production by in vitro transcription and/or in the use of cell-free systems will be highly appreciated
- strong organizational skills, ability to make proposals, ability to integrate into a collaborative project
- oral and written communication skills in both French and English.
The host team is located at CEA Saclay (20 km southwest of Paris) and is part of the Institute of Integrative Cell Biology (I2BC, UMR 9198 CNRS, CEA, Université Paris Saclay) (https://www.i2bc.paris-saclay.fr/spip.php?article1179).
The team is composed of 3 researchers and 1 senior technician. It regularly welcomes M2 students, PhD students and post-docs. The team works on the biological synthesis of natural products and uses synthetic biology approaches to produce improved derivatives of these natural products.
The candidate may be required to spend short periods of time in one of the partner laboratories (Synthelis).
Commentaire : 12-month fixed-term renewable for 24 months
Please apply directly through this system
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