Postdoctoral Researcher (M/F) in Cell Imaging and Lipid Trafficking

Job Description

Missions

The recruited researcher will investigate the molecular mechanisms involved in cholesterol transport and storage at endoplasmic reticulum–plasma membrane contact sites, as well as their relationship with lipid droplet metabolism. They will use cell and molecular biology approaches, live-cell imaging by confocal and TIRF microscopy, lipid visualization techniques, and electron microscopy. They will be responsible for data acquisition and quantitative analysis, interpretation of results, and dissemination of findings through scientific presentations and publications.

Activity

1. Conduct cell and molecular biology experiments to investigate the role of membrane contact sites in cholesterol transport and storage.
2. Study protein dynamics and localization at membrane contact sites using live-cell imaging, including confocal, TIRF and super-resolution microscopy.
3. Visualize and analyze the intracellular distribution of lipids, particularly cholesterol, and their transport between cellular compartments.
4. Use electron microscopy to characterize cellular ultrastructure, contacts between organelles, and lipid droplet morphology.
5. Perform experimental data acquisition, quantitative analysis and interpretation.
6. Present results at scientific meetings and contribute to the preparation of scientific publications.
7. Participate in scientific discussions and collaborative activities within the research team.

Your Profil

Skills

1. PhD in cell biology.
2. Proven experience in live-cell imaging using confocal, TIRF and super-resolution microscopy.
3. Experience in electron microscopy and analysis of cellular ultrastructure.
4. Skills in lipid visualization and the study of intracellular lipid trafficking.
5. Knowledge of membrane contact sites and lipid transport mechanisms.
6. Proficiency in cell and molecular biology techniques.
7. Skills in quantitative image analysis and experimental data processing.
8. Ability to work independently, scientific rigor, analytical skills and ability to work as part of a team.
9. Strong scientific communication and scientific writing skills.
10. Proficiency in written and spoken scientific English.

Your Work Environment

The project will be carried out within the Lipid Trafficking and Membrane Contact Sites team, led by Francesca Giordano in the Cell Biology Department at I2BC. The team specializes in lipid biology, membrane contact sites and advanced imaging. The project will benefit from the laboratory's equipment and access to the Imagerie-Gif core facility, particularly for fluorescence and electron microscopy. The project is funded by the French National Research Agency (ANR).

Constraints and risks

Experimental activities require compliance with laboratory safety regulations, particularly for cell culture and the handling of chemical reagents. The use of specialized imaging equipment requires appropriate training. Experimental protocols and procedures for the prevention of biological and chemical risks must be followed.

Compensation and benefits

Compensation

Monthly gross salary between2 991,58 € and 4 756,76depending on experience

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

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