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Portail > Offres > Offre UMR9197-ODILEC2-118 - Post-Doctorant en neurobiologie du vieillissement (H/F)

-Post-Doctorant in neurobiology of aging (M/F)

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : lundi 10 octobre 2022

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General information

Reference : UMR9197-ODILEC2-118
Workplace : SACLAY
Date of publication : Monday, September 19, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 December 2022
Proportion of work : Full time
Remuneration : -salary between €2,889 and €4,541 gross per month, depending on professional experience and CNRS salary scale
Desired level of education : 5-year university degree
Experience required : Indifferent

Missions

In the context of an ANR project (Aging-jou), the Post-Doctorant will participate to the characterisation of the role of a cluster of 3 snoRNAs, including jouvence, in the determination of longevity, aging, and neurodegenerescence, in Drosophila.

Activities

- Genetic of Drosophila: P[Gal4] system, RNAi, mutants, double mutants, CrispR/Cas9
- Quantification of metabolic parameters (metabolomic): triglycerides, cholestérol
- Physiological analysis: Longevity
- Molecular Biology analysis of the differents mutants (Crisp/Cas9).
- Transcriptomic analysis (RNA-seq) & proteomic
- in-situ Hybridization
- Biochemistry : analysis of the pseudouridylation and its consequences
- Analysis of the traduction (Ribosome & Polysome profiling)
- Histology & immunohistology on Drosophila brain.
- Diffusion & Valorisation of the results, such as oral presentations and publications

Skills

Skills:
- Biology : applied méthodology (depp skill)
- Knowledges in health and safety rules
- Applied Informatic
- English language : B2 to C1 (european reference)

Operational skills
- Designing experimental devices (expert)
- Developing scientific and technological expertise
- Use activity-specific software (mastery)
- Writing scientific documents (expert)

Behavioural competencies
- Analytical thinking skills
- Organisational skills
- Interpersonal skills

Work Context

Recently, in Drosophila, we have identified a new small nucleolar RNA (snoRNA) (jouvence) and showed that its mutation (deletion) reduces lifespan, while its overexpression increases it. At the organismal level, the old mutant flies show more neurodegenerative lesions than the controls, while the flies overexpressing jouvence show only few lesions, suggesting a neuroprotection. The mutants present also a hypertrophy of the fat body, suggesting a perturbation of the lipids metabolism. jouvence is required in the epithelium of the gut (1,2). However, recently, a deeper characterization of this genomic locus has shown the presence of two other snoRNAs, revealing rather a cluster of three snoRNAs. The objectives of this project are to determine the role of each snoRNAs, and more particularly the longevity, the neurodegenerative and intestinal lesions, as well as the metabolic perturbations (gut-brain axis) of each snoRNA mutant. the agent will be placed under the hierarchical responsibility of the team leader Mr. Jean-René MARTIN.

Additional Information

Possibility of contract extension

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