Missions

The position, funded by the National Cancer Institute (INCa) is offered within the laboratory of Dr. Beatrice Rondinelli at the Genome Integrity and Cancers Unit (UMR 9019, www.gustaveroussy.fr/en/umr-9019) at Gustave Roussy Institute, located just outside of Paris city center.
The main mission is to study the contribution of chromatin to DNA damage repair. This involves deepening the understanding of chromatin-related factors and pathways that stabilize and repair damaged replication forks (RFs) in human cells. Bioinformatic analysis of proteomic and drug sensitivity datasets, followed by wet lab validation, has allowed the identification of chromatin-related factors contributing to the RF damage response, whose mechanism is currently uncharacterized. The goal of this project is to use biochemistry and molecular biology approaches to unveil the functional interaction between the chromatin enzyme of interest and key, interacting DDR factors.

Activités

The candidate's core activities will involve the generation of mutants cDNA and stable cell lines, essential for experimental manipulation and study. They will also conduct in vitro methylation and interaction assays to investigate molecular mechanisms. Furthermore, the role will require performing various DNA repair assays, including specific methods like comet and native ssDNA assays, alongside other state-of-the-art DNA repair and genome integrity assays.
The main activities include:
• Generation of mutants cDNA and of stable cell lines;
• In vitro methylation and interaction assays;
• DNA repair assays such as comet, native ssDNA and other state-of-the-art DNA repair/genome integrity assays.

Compétences

We invite highly motivated and dynamic candidates, capable of working independently while being good team players. The candidate must have solid experience in cellular and molecular biology. Working experience in the fields of chromatin organization or DNA damage response will be appreciated. The candidate must be proficient in English.

Contexte de travail

The position is offered within the laboratory of Dr. Beatrice Rondinelli at the Genome Integrity and Cancers Unit (UMR9019), at Gustave Roussy Institute, located just outside of Paris city center. Gustave Roussy Institute is the leading cancer center in Europe, focusing on the study of cancer biology and treatment.
The host "Genome Integrity and Cancers" unit offers a stimulating environment and dedicated equipment for research in the DNA repair and replication fields. The Institute also provides access to state-of-the-art facilities, including cell imaging, genomics, animal housing, and bioinformatics.
Initial funding is offered for a 12-month contract with Gustave Roussy, with possible extension. The successful candidate will be encouraged to apply for French and international grants.