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Reference : UMR9019-ALEISH-003
Workplace : VILLEJUIF
Date of publication : Tuesday, May 19, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 15 July 2020
Proportion of work : Full time
Remuneration : 2695 gross salary per month
Desired level of education : PhD
Experience required : 1 to 4 years
A post-doctoral Research Scientist position is open in the field of Molecular biology, DNA damage response and repair in the team “Genetic and Epigenetic Repair” led by Alexander ISHCHENKO at the Institute Gustave Roussy (UMR9019 ex8200 CNRS/Université Paris Saclay, Villejuif, France – https://www.gustaveroussy.fr/en/node/1811). Recent studies in the host lab showed a novel type of activity of mammalian PARPs proteins that can directly attach mono- or poly(ADP-ribose) (MAR or PAR, respectively) moieties not only to proteins but also to the DNA termini at the sites of DNA strand breaks. It is generally accepted that PARP-mediated ADP-ribosylation of proteins play important role in DNA strand breaks repair, synthetic lethality and chemoresistance but theirs mechanisms are still elusive.
This project will focus on the mechanisms of PARPs-mediated DNA PARylation, identification proteins responsible for ADP-ribose-DNA adduct recognition, the role of DNA ADP-ribosylation in non-homologous end joining; establish a relationship between DNA ADP-ribosylation, genotoxic stress and acquired chemoresistance of cancer cells. The new knowledge about the mechanisms and the role of DNA modification by PARPs in the cellular response to DNA damage will identify novel therapeutic or diagnostic targets in cancer and other age-related diseases.
The successful candidate will work under the scientific direction of A. ISHCHENKO, and will:
- participate in the design of the project;
- use and develop methodologies (based on classical techniques of biochemistry (70%), molecular and cellular biology (30%) ;
- carry out experiments (purification of recombinant proteins, in vitro and ex vivo DNA repair reconstitution, immunocytochemistry, antibody production, quantitative proteomics etc.);
- analyze and communicate the data,
- write the publications.
The candidate must have a Ph.D. in Biochemistry or Biology, and have solid experience in molecular biology and biochemistry techniques. A good knowledge of the DNA repair mechanisms and/or PARPs fields will be appreciated. The candidate must be highly motivated, autonomous, interactive, rigorous and curious, have excellent organizational and teamwork skills, the ability to lead, and be able to express himself / herself in English, written and spoken.
The Institute Gustave Roussy is one of the world's leading cancer-research institutes and the premier European Cancer Centre. It has a multidisciplinary work environment and joins together 12 research units (CNRS / Inserm) conducting basic, clinical and translational research. The postdoctoral researcher will benefit from an extremely stimulating scientific environment of the UMR 8200 “Genetic stability and oncogenesis” unit, and will have access to core facilities, located at the Gustave Roussy
Constraints and risks
-Work with 32P radioactively labelled DNA constructs. Radiation protection training will be provided by the Gustave Roussy.
The contract is financed by the ANR (National Research Agency). Applications must be submitted on emploi.cnrs.fr site; they should include a CV and a letter of motivation summarizing the scientific interests of the candidate, as well as the contact information of 2 referees who could provide recommendations. Additional information can be obtained by email to Alexander.ISHCHENKO@gustaveroussy.fr
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