Reference : UMR9004-CORBEZ0-006
Nombre de Postes : 1
Workplace : MONTPELLIER
Date of publication : Wednesday, January 11, 2023
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 15 February 2023
Proportion of work : Full time
Remuneration : 2833 euros bruts mensuels
Desired level of education : PhD
Experience required : Indifferent
Manipulation of cytosolic sensors by the stealth bacterial pathogen Coxiella burnetii
Our laboratory focuses on host/pathogen interactions occurring during infection with the intracellular bacterial pathogen Coxiella burnetii, the causative agent of the zoonosis Q fever. This stealth bacterium can invade and develop in a wide range of cell types, such as macrophages and trophoblasts. Coxiella possesses a Dot/Icm Type 4b Secretion System (T4SS) that allows the secretion of bacterial effector proteins. These effectors participate in the formation of an intracellular replicative niche named Coxiella-Containing Vacuole (CCV) as well as the inhibition of apoptosis. Our laboratory and others have shown that Coxiella can dampen the inflammatory response of infected cells to promote intracellular persistence (Mahapatra et al., 2016, Burette et al., 2020, Cunha et al., 2015). Recently, we have identified additional effectors capable of interacting with several cytosolic sensors of the NOD-like receptors family (NLRs), which play a crucial role in the recognition of intracellular pathogens and the inflammatory response of immune and placental cells.
The role of the candidate will be to characterise these newly identified effector proteins and determine how Coxiella manipulates host NLRs to evade the innate immune response. This is an exciting opportunity to better understand Coxiella's stealth strategies of infection.
- Bacteria and cell culture
- Molecular and cellular biology techniques: cloning, ectopic expression, qPCR, immunoprecipitation, Western blot analysis, immunofluorescence, ELISA
- Microscopy: bright field, fluorescence, confocal
- Flow cytometry
- Common laboratory duties: buffer preparation, stock management
- Literature analysis
- PhD student co-supervision
- Scientific article writing and editing
- Technical skills in cellular microbiology and immunology
- Communication skills in English (written and oral)
- Team spirit
The laboratory is composed of 10 scientists (Senior, junior, lecturer, engineers and PhDs) and is hosted in the Infectiology Research Institute of Montpellier (France), which is supported by the CNRS and the University of Montpellier. The Institute is composed of 11 teams working at the forefront of research on bacterial and viral diseases. It provides an exciting, multi-disciplinary and multi-cultural environment, and is connected to state-of-the-art facilities in microbiology (BSL2 and BSL3), microscopy, cytometry and mass spectroscopy.
Constraints and risks
The candidate will work in BSL2 environments.
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