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Reference : UMR9002-DOMMAI-003
Workplace : MONTPELLIER
Date of publication : Friday, September 06, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 November 2019
Proportion of work : Full time
Remuneration : 2586 € gross per month max (depending on experience)
Desired level of education : PhD
Experience required : 1 to 4 years
The aim of the project is to study at the molecular level the functional interaction between the transcription and replication machineries at the level of DNA / RNA structures (R-loops). The study will focus on the role of the Ddx19 RNA helicase, which we have recently shown to be required for R-loop resolution (Hodroj et al., EMBO J. 2017), in this process. The mission of the candidate will be to study the molecular interactions of Ddx19 with the R loops and chromatin and to study the subcellular localization of Ddx19 after DNA damage- and/or oncogenes-induced replicative stress.
-Cloning and amplification of DNA by PCR
-Protein and chromatin immunoprecipitation from cell lysates
-Expression and purification of recombinant proteins
-Tests of enzymatic activity in vitro
Trainee supervision (master level)
A very good experience in biochemistry, molecular and cellular biology is required.
-Motivation and passion for science.
-At least one publication of good level (IF> =8) as the first author resulting from the work of thesis.
-Capacities of organization and communication
- Ability to work in a team
- Knowledge of basic techniques of molecular and cellular biology (western blot, PCR, cloning, immunolabeling).
- Mammalian cell culture
- Chromatin Immunoprecipitation
The Institute of Human Genetics offers an exceptional scientific environment with 22 research teams. One of the major areas of research developed at the institute is genome dynamics and epigenetic control. The main aspects of this research concern DNA replication, maintenance of genome integrity, recombination and meiosis, chromatin and nuclear organization.
Constraints and risks
No particular risks or constraints
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