Reference : UMR8612-SIMMUR-013
Workplace : ORSAY
Date of publication : Thursday, September 15, 2022
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 November 2022
Proportion of work : Full time
Remuneration : between 2890 and 3322 € gross monthly
Desired level of education : PhD
Experience required : 1 to 4 years
The preclinical development of cancer therapies generally involves the evaluation of potential anticancer agents on tumor cell cultures in vitro. The poor performance in terms of predicting therapeutic responses in humans from available in vivo or in vitro models has motivated the search for alternative in vitro models that are truly predictive of human cancer. A recent approach to in vitro cancer modeling is based on the development of organs on microfluidic chips associated with the culture of spheroids allowing to recapitulate the multicellular architecture, the tissue-tissue interfaces, and the physiologically relevant physical microenvironment of cancers in the human body. man. In this context, the TumFlu project funded by the LABEX LaSIPS proposes to manufacture and optimize a dedicated microfluidic device then to undertake the culture of tumor spheroids in the microfluidic system and finally to study the role of the tumor microenvironment (stiffness, oxygenation) on the proliferation and cell migration and response to treatments.
• Design and fabrication of a microfluidic device
• Construction of a 3D tumor model
• Optimization and validation of cell culture in the microfluidic device
• Understand how environmental conditions (i.e., biochemical, and mechanical clues) will affect tumor progression and metastatic capacity.
Analysis of results and preparation of progress reports.
Essential skills in micro-engineering and microfluidics (photolithography, microfluidic device fabrication processes, flow control in biochips, etc...)
Highly desirable skills in cell biology, bioassays, molecular biology, confocal imaging, in vitro cell immunology techniques, immunohistochemistry and immunofluorescence techniques
Knowledge of 3D culture methodologies, and/or tumor-microenvironment interactions is also an asset
The project will take place mainly within the CNRS C2N laboratories (Centre for Nanosciences and Nanotechnologies) and the Institut Galien Paris-Saclay of Paris-Saclay University in close collaboration with the CNRS LMPS laboratory (Laboratory of Mechanics Paris-Saclay), CentraleSupélec, Paris-Saclay University within the framework of the LABEX LaSIPS TumFlu project.
Paris-Saclay University is located about 25 km south of Paris on the plateau de Saclay.
Constraints and risks
No specific constraints
The eligible candidate must be within 2 years of his/her PhD thesis defense
PhD students scheduled to defend in the October 2022 -January 2023 time frame may also apply.
To carry out this interdisciplinary project the candidate should possess solid skills in microengineering and microfluidics (e.g., photolithography and microfluidic fabrication processes, flow control in biochip, etc…) and validated experience in cell culture and biological assays as demonstrated by the publication record, including at least one first authorship.
The candidate must be proficient in oral and written English.
We are looking for candidates interested to work in a multidisciplinary research environment, who have excellent communication skills and are team-oriented, focused, self-motivated, trustworthy, critical and open-minded.
We exclusively accept applications submitted through our online application portal. Applications via email will not be considered.
The application must be in English and include a curriculum vitae with names and contact information of 3 referees, a complete list of publications and a statement of research interest. The application documents must be sent as a single PDF file. Incomplete applications will not be considered for evaluation.
Applications will be reviewed as they are received until the position is filled.
For inquiries about the position, please send an email to Dr. Simona Mura (email@example.com), Dr. Bertrand David (firstname.lastname@example.org), Dr. Anne-Marie Haghiri (email@example.com) with the subject line ""LabexLaSIPS_postdoc"
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