Missions

The post-doctoral student will be involved in the ANR CAMOMILL project, interacting with the other project partners. His/her role will be to develop multi-modal microscopy tools and methods for cross-analysis of FCS (Fluorescence Fluctuation) and SPT (Single Molecule Tracking) data. Together with a PhD student, he will be involved in applying these tools to monitor the dynamics of the proteins responsible for regulating ARNP II.

Activities

-Work on fluorescently labelled living cells
- Measurement of molecular dynamics and interactions by FCS, SPT and FRET.
- Instrumentation: Setting up a multiplex measurement device - Carrying out experimental campaigns
- Analysis of data and development of associated methods
- Contributing to the development of modelling methods
- Presenting results at team meetings and conferences
- Publications

Skills

- Autonomy and organisation
-Ability to work in a team
- good knowledge of optics
- Autonomy in microscopy
- mastery of image analysis techniques
- Basic knowledge of cell biology.

Work Context

The Functional Cellular Biophotonics group (Resp. L.Heliot) of the Physics of Lasers, Atoms and Molecules laboratory (PhLAM) has been developing original techniques for molecular imaging of living cells using fluorescence microscopy for several years within the DySco team. This interdisciplinary group (biology, instrumentation, physics, image analysis) is developing techniques to analyse and quantify molecular interactions and dynamics. Our research focuses on the study of molecular assembly dynamics within regulatory networks. The post-doctoral student will be working on the ANR CaMoMiLL project. This project is being carried out in collaboration with the teams of Aymeric Leray (Lab. Carnot, Dijon); Jean-Baptiste Courbot (IRIMAS, Mulhouse). The aim of this project is to study the dynamics of RNA polymerase II (RNAP II) and its spatiotemporal regulation in the nucleus. We will measure the movement of RNAP II and regulatory factors in the nucleus on disjoint spatiotemporal scales (FCS and SPT). This requires the development of multimode microscopes that couple the two measurements. The measurements will be analysed using approximate Bayesian computation (ABC) and artificial intelligence (DL) algorithms. The parameters deduced will provide us with a multi-scale quantification of the experimental data. We will study the dynamics of ARNP II under controlled or perturbed conditions (genetic mutants, RNA interference, pharmacological drugs) in order to understand the regulatory mechanisms and their remodelling under conditions of stress, as well as the mechanisms regulating the circadian clock.