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Portail > Offres > Offre UMR8520-ALEVLA-001 - Postdoctoral Researcher (H/F) on Droplet microfluidics, molecular programming and high throughput enzyme screening

Postdoctoral Researcher (M/W) on Droplet microfluidics, molecular programming and high throughput enzyme screening

This offer is available in the following languages:
- Français-- Anglais

Date Limite Candidature : jeudi 21 mars 2024

Assurez-vous que votre profil candidat soit correctement renseigné avant de postuler

Informations générales

Intitulé de l'offre : Postdoctoral Researcher (M/W) on Droplet microfluidics, molecular programming and high throughput enzyme screening (H/F)
Référence : UMR8520-ALEVLA-001
Nombre de Postes : 1
Lieu de travail : VILLENEUVE D ASCQ
Date de publication : vendredi 22 décembre 2023
Type de contrat : CDD Scientifique
Durée du contrat : 12 mois
Date d'embauche prévue : 1 février 2024
Quotité de travail : Temps complet
Rémunération : between 2 135,35 € and 4 541,08 €
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : Indifférent
Section(s) CN : Micro and nanotechnologies, micro and nanosystems, photonics, electronics, electromagnetism, electrical energy

Missions

The postdoctoral researcher will be in charge of droplet generation for beads synthesis, hydrogel chemistry, microfluidic chip design for beads encapsulation in molecular program droplet.

Activités

She/He will be in charge of setting up the microfluidic setup. She/he will be in charge of performing the experiments, analysing the data, presenting the data to group members and consortium members.

Compétences

Microfluidics (droplets an advantage), Chemistry, Enzyme (an advantage), Microfabrication

Contexte de travail

The transition of our societies towards greater sustainability will increasingly rely on the enzymatic transformation of renewable biomass into high-value commodities. Yet, this step remains difficult on lignocellulose (LC) due to its high degree of recalcitrance, which involves specific physical processes which are often ignored when searching for efficient enzymes.
Ultra-high-throughput activity screenings (uHTS) offer nowadays the possibility to explore immense enzyme diversity, a necessity to find adequate LC active enzymes especially when they are used in combination. Alas, uHTS are currently based on soluble fluorogenic substrate analogs limiting the relevance of their results.
In this project we propose to implement a novel droplet microfluidic uHTS approach which associates a molecular programming method to the production of micron sized hydrogel beads to emulate complex substrates. We aim to achieve single enzyme sensitivity at 10^8/h throughput, making possible the discovery of must-find enzymes active on recalcitrant substrates. Moreover, by relying on the unique versatility of molecular programming, we will develop an approach able to perform the selection of the best enzyme without having to sort droplets and analyse their content.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.