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Portail > Offres > Offre UMR8246-JOCCAB-004 - CDD chercheur expérimentation biologique H/F

-Biological experimentation researcher M/F

This offer is available in the following languages:
Français - Anglais

Date Limite Candidature : vendredi 2 septembre 2022

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General information

Reference : UMR8246-JOCCAB-004
Workplace : PARIS 05
Date of publication : Friday, July 22, 2022
Type of Contract : FTC Scientist
Contract Period : 7 months
Expected date of employment : 17 October 2022
Proportion of work : Full time
Remuneration : constraints include: Some weekends for behavioral studies.
Desired level of education : 5-year university degree
Experience required : 1 to 4 years

Missions

Under the supervision of the SNRG team leaders (https://www.ibps.sorbonne-universite.fr/fr/Recherche/umr-8246/signalisation-neuronale-et-regulations-geniques), the post-doc will carry out a series of experiments to determine the antidepressant properties of new molecules in preclinical models of depression in mice. He/she will study post-mortem samples of brain tissue of interest and blood samples will be taken to study biomarkers of depression and their regulation by antidepressant treatments by Q-RT-PCR.
The post-doc will be in charge of behavioral experiments, their quantitative measurements and their interpretation. He/she will perform immunohistochemical and FISH (fluorescent In Situ Hybridization) experiments on post-mortem brain sections, as well as assays on blood extracts. He/she will perform image acquisition on a confocal microscope as well as data analysis, graphic and quantitative representation.

Activities

- Plan and perform behavioral studies in mice (chronic social stress).
- Perform brain sections after intracardiac infusions of fixative solution (PFA).
- Perform immunohistochemical and morphological studies on post-mortem brain sections.
- Perform mRNA measurements by Q-RT-PCR on brain and peripheral extracts.
- Acquire images by confocal microscopy.
- Analyze data with ad'hoc software.
- Write quantitative tables and graphs
- Carry out statistical tests.

Skills

- Solid theoretical and practical knowledge of animal behavior (level 1 animal experimentation certificate)
- Theoretical and practical knowledge of immunohistochemistry and imaging on post-mortem brain sections
- Practical knowledge of image acquisition techniques using confocal microscopy
- Practical knowledge of software dedicated to imaging fields (Metamorph, ImageJ FlowJo...)
- Interpersonal skills: listening skills and adaptability to interact with team members and the scientific consortium.
- Theoretical and practical knowledge in biology
- Theoretical and practical knowledge in statistics

Work Context

The SNRG (Neuronal Signaling and Gene Regulation) team of the Neurosciences Paris Seine laboratory specializes in the study of behavioral and neuronal adaptations in mouse models of psychiatric disorders (depression, addiction, Huntington's disease) (https://www.ibps.sorbonne-universite.fr/fr/actus/publications?t=t153e9df32cab05f).
Peter Vanhoutte and J. Caboche are pioneers in the study of intracellular MAPkinase/ERK signaling pathways in the central nervous system and their role in memory and cognitive processes, particularly in addiction and depression.
In the framework of a short collaboration, the team is recruiting a post-doc researcher who will take charge, under the responsibility of the team leaders, and of the associated researchers in the laboratory, of a set of experiments aiming at studying the preclinical properties of a compound targeting the ERK pathway in depression. Overall, this study will lead to a new therapeutic strategy for the treatment of depression.
The areas of expertise within the team cover many fields: from cellular and molecular studies to studies of complex motor and cognitive behavior in mice. Our skills also include expertise in histology and cellular imaging on post-mortem brain sections in humans.
The means at our disposal include: a behavioral phenotyping platform, an immunohistology laboratory, a cell imaging platform: confocal laser scanning microscopes, workstations (Huygens, Volocity, imageJ), computer and image analysis software.

Constraints and risks

constraints include: Some weekends for behavioral studies.

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