Missions

The Nanobio team is developing new single molecule localisation microscopy methods to push back the limits of these observations in terms of both acquisition speed and depth of observation. In particular, the team has demonstrated the benefits of introducing structured excitation for the observation of complex samples such as tissue or organoids. The aim of this post is to develop a new implementation of this technique called ModLoc for modulated localization. The aim is to set up a new instrument that will improve stability, resolution, acquisition speed and observation volume. In particular, the sample excitation and fluorescence detection methods will be rethought, then validated on various deep biological applications (several tens of microns). This project will be carried out as part of the EIC Nanoscan, which includes Politechnico di Milano, the CNS, Laserlab and abbelight, and will benefit in particular from a new generation of patented optical chips for generating structured excitation.

Activités

The person will contribute to various aspects :
- setting up the new excitation/detection for this new instrumental device
- testing and validation of the new experimental set-up
- pilot/simulation programming
- characterisation of various samples
- writing articles
- taking part in conferences

Compétences

- optics
- microscopy
- knowledge of single-molecule imaging (acquisition and processing)
- knowledge of photophysics
- knowledge of cell culture
- python programming

Contexte de travail

This work will be carried out within ISMO (Unité Mixte CNRS / Université Paris-Saclay) as part of a European EIC Transition Nanoscan project involving 2 other laboratories, the european network Laserlab and one company (Abbelight). The laboratory has an on-site culture room and various microscopes for single molecule localization microscopy, SIM and FLIM.

Contraintes et risques

laser beam
culture cell