Informations générales
Intitulé de l'offre : Post-doctoral fellow (M/W) for the development of a new concept in localisation microscopy of individual fluorescent emitters (H/F)
Référence : UMR8214-SANLEV-018
Nombre de Postes : 1
Lieu de travail : ORSAY
Date de publication : jeudi 14 septembre 2023
Type de contrat : CDD Scientifique
Durée du contrat : 24 mois
Date d'embauche prévue : 1 décembre 2023
Quotité de travail : Temps complet
Rémunération : Gross monthly salary depending on experience: For a researcher with less than 2 years experience after the PhD: 2889,51 € between 2 and 7 years experience: 4082,90€
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : Indifférent
Section(s) CN : Atoms and molecules, optics and lasers, hot plasmas
Missions
The Nanobio team is developing new concepts to push back the limits of lsingle molecule localization microscopy. This position will involve developing a new concept for the localisation of individual fluorescent emitters, based solely on a temporal approach and no longer on spatial analysis of the emission spot. This will make it possible to completely rethink the whole localisation strategy, speed up acquisition, overcome the obstacles in terms of in-depth observation, etc. In particular, the aim will be to design new optical implementations for setting up a chirped structured excitation (in 2D then 3D) offering uniqcity between the position and frequency of the detected signal, making it possible to use a single detector alone or in a matrix to analyse a wide field of observation. The aims of this project are to make it possible to observe/trace single molecules at previously unattainable acquisition rates, and also to open up new areas of observation in spectroscopy, by combining position information on a nanometric scale with detection of the spectrum or lifetime of fluorescence. These new developments are based on an initial patented proof of concept, and will be implemented as part of the TimeNanoLive ERC.
The post may be extended for a further 12 months.
Activités
The person will contribute to various aspects :
- setting up the optical implementations of this new concept
- testing and validation of the new experimental set-up
- pilot/simulation programming
- characterisation of various samples
- writing articles
- taking part in conferences
Compétences
- optics
- microscopy
- knowledge of single-molecule imaging (acquisition and processing)
- knowledge of photophysics
- knowledge of cell culture
- python programming
Contexte de travail
This work will be carried out within ISMO (Unité Mixte CNRS / Université Paris-Saclay) as part of a European proejct ERC TimeNanolive ). The laboratory has an on-site culture room and various microscopes for single molecule localization microscopy.
Contraintes et risques
laser beam
culture cell