M/F Postdoctoral Position in Molecular and Cellular Neuroscience – Study of the Activation Mechanisms of the Atypical Glutamate Receptor GluD1

Job Description

Missions

Our group studies a key family of receptors in the nervous system: ionotropic glutamate receptors. We are particularly interested in the structural and functional diversity of this receptor family and its impact on normal and pathological neuronal communication. We have recently turned our attention to the delta family of receptors (GluD receptors), which are atypical members of the glutamate receptor family due to their insensitivity to glutamate and their inability to open their channel. The postdoctoral fellow will be tasked with investigating the molecular mechanisms underlying signal transduction in these receptors. To do so, the candidate will use tools developed in the laboratory to study GluD receptors: (i) electrophysiological tools based on the use of constitutively open GluD mutants, as well as (ii) fluorescence tools that allow real-time tracking of conformational changes in specific regions of the receptor (voltage-clamp fluorometry). The postdoctoral fellow will also perform molecular dynamics simulations to interpret the experimental data from a structural perspective.

Activity

- Electrophysiology on dissociated cells (Xenopus oocytes)
- Voltage-clamp fluorometry
- Voltage-clamp fluorometry
- Biologie moléculaire, biochimie
- Molecular biology, biochemistry
- Protein engineering
- Molecular modeling
- Treatment and maintenance of Xenopus oocytes
- Conduction of an experimental project
- Data analysis
- Formatting and presenting results
- Publication
- Participation in the laboratory's common tasks

Your Profil

Skills

- Skills in electrophysiology
- Advanced skills in molecular biology and protein engineering
- Basic knowledge of chemistry and molecular neuroscience
- Advanced knowledge of structure-function investigations on receptors/membrane proteins
- Ability to work independently and organize one's work
- Adaptability, reliability, and experimental rigor
- Teamwork
- Training in animal experimentation specializing in aquatic animals and/or initial training in experimental surgery is a plus.

Your Work Environment

The postdoctoral fellow will work in the “Glutamate Receptors and Excitatory Synapses” team at the Institute of Biology of the École Normale Supérieure (IBENS, CNRS UMR 8197, INSERM U1024, ENS-PSL University). This interdisciplinary institute, located within a dense network of laboratories in central Paris (ENS-PSL, Institut Curie, Collège de France, ESPCI Paris-Tech, etc.), benefits from an exceptional research environment. The candidate will work independently in close collaboration with Laetitia Mony, the principal investigator. This research project will be carried out with funding from the ANR.

Compensation and benefits

Compensation

According to experience between 3 131 € and 4 806 €

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions