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Reference : UMR8197-NATBOI-022
Workplace : PARIS 05
Date of publication : Tuesday, August 13, 2019
Type of Contract : FTC Scientist
Contract Period : 24 months
Expected date of employment : 1 January 2020
Proportion of work : Full time
Remuneration : Between 2695 and 3840 euros gross salary based on experience
Desired level of education : 5-year university degree
Experience required : 1 to 4 years
The project developed at IBENS aims to understand the evolutionary distribution and environmental functions of proteins found in chloroplasts throughout the eukaryotic tree of life. This will be used to better understand how different algae adapt to their environment, either through long-term phenomena that influence evolutionary processes or by mechanisms of adaptation and acclimatization in the shorter term; and to identify new proteins that contribute to the success of algae that could potentially be used to increase the photosynthetic productivity of terrestrial plants. The project involves the proteomic characterization of chloroplasts isolated from marine algae, notably dinoflagellate Amphidinium, which will be supplemented by the development of computer tools informed by experimental data. At the same time, the candidate will use a simple but powerful biological system, the model diatom Phaeodactylum tricornutum, to understand the links between the proteome of chloroplasts and the adaptation of algae to environmental conditions, for example by creating transgenic lines mutated for interesting proteins.
The candidate will deal with two experimental projects: first, the development of biochemical techniques for the purification of chloroplasts, or the enrichment of these organelles with a centrifugation gradient, with the dinoflagellate model Amphidinium. These cell extracts will be used for mass spectrometry, either in conventional form or in quantitative form ("LOPiT"), with the support of Prof. Kathryn Lilley and Prof. Ross Waller (Cambridge Univ.), To better understand the contents of this organelle in dinoflagellates. The candidate will thus be able to develop computer tools to predict how proteins are sent to chloroplasts in other dinoflagellate species, to better understand the diversity of metabolic pathways hosted in chloroplasts. The second project will be concerned with the functional characterization of the proteins found only in the chloroplasts of algae originating from secondary endosymbiota, which do not exist in plants. The candidate will produce his own lines of key protein mutants in the diatom Phaeodactylum, by CRISPR-CAS9; identify successful mutant lines by high-throughput genotyping; and finally characterize its mutants, by flow cytometry, photo-physiology, and transcriptomics. The candidate will participate in the choice of genes to target. The candidate will have the opportunity to create mutants in two other species, Cyclotella (a diatom that is optionally non-photosynthetic) and Nannochloropsis (a species of eustigmatophyte rich in natural oils), in collaboration with Prof. Angela Falciatore (IBPC, Paris) and Prof. Hanhua Hu (Wuhan University, China).
The candidate must:
- have competence in cell culture (prokaryotes and / or eukaryotes) under laminar flow hood
- have a good training in molecular techniques (PCR, qPCR, reverse transcription ...)
- have experience in protein analysis, ideally western blot, immunoprecipitation, and preparation of protein samples
an experiment in the separation of organelles by gradient centrifugation will be a more appreciable
- a basic training in the quantitative analysis of data (R, MatLab ...) and one or two programming languages (Perl, Python) would also be desired but is not required
- be rigorous and organized in order to carry out several activities in parallel
- be prepared for discussions in English
Level of recruitment: Post-doc.
The Plant & Diatom Genomics hosting team offers a user-friendly work environment led by Chris Bowler (ibens.ens.fr). It includes 3 PhD students, 9 post-docs, 2 teacher-researchers and 2 CNRS Research Directors. The team's work focuses on the mechanisms by which the environment can influence the structure and dynamics of genomes as an adaptive mechanism in the course of life and evolution of photosynthetic species. The candidate's daily activities will be led by Dr. Richard Dorrell, post-doc in the team, and recipient of a three-year CNRS grant to complete this project.
The Institute of Biology of the Ecole Normale Supérieure (IBENS) is a multidisciplinary research center with international vocation located in the Latin Quarter in the center of Paris, close to many means of transport (RER, metro, bus). This mixed CNRS / Inserm / ENS unit comprises around 320 staff, divided into 29 research teams and supports the employment of minorities.