Post-Doctoral fellow (M/F) in Immunology

Job Description

Missions

Plasmacytoid Dendritic Cells during HIV-1 or HIV-2 infections: targets for "HIV-Cure"

People living with HIV-1 cannot interrupt their anti-retroviral treatment without rebounding their viral loads. HIV-2 compared to HIV-1 infection progresses more slowly, and represents a model for research on HIV-cure (i.e., spontaneous control by the immune system, allowing anti-retroviral treatment interruption).
Plasmacytoid dendritic cells (pDC) are central to the difference between HIV-1 and -2 infection outcomes, because they respond to viruses by producing interferons and by stimulating adaptive immune responses. We discovered that pDC stimulated by HIV-1 or -2-infected cells evolve into several sub-populations with different functions (Khelfa art in prep).
The aim of this project is to study the respective functions of these subpopulations, to compare frozen pDC previously collected from different ANRS cohorts (CO5-Immunovir 2, Codex, HIV-IFN) of people living either with or without HIV-2 or HIV-1, with either disease progression or elite control; and to seek immune targets to develop new immune therapies toward "HIV-Cure".

Activity

The project implies pDC and sub-population purification, preparation of the cells for sequencing (encapsulation and sequencing done by the Genom'IC facility), result analysis (R-studio, with the help of the Bioinformat'IC facility), functional studies by cell culture, Aurora spectral cytometry, MSD analyte dosage, immunofluorescence, and modulating subpopulation in vitro by potential therapeutic agents.

Your Profil

Skills

• Immunology, including if possible dendritic cells or IFN or HIV
• Cell culture and isolation, cellular immunology, flow cytometry
• Bioinformatics (R )
• Analytical reasoning ability, organization skills, team spirit
• 0 to 7 years experience after PhD

Your Work Environment

Institut Cochin is a biomedical research center located in the center of Paris (22 rue Méchain),, 75014 Paris). It is affiliated to INSERM, CNRS and Université Paris Cité. It is composed of 35 teams and 10 core facilities. Work conditions are attractive (multidisciplinary research, seminars, Jecco young researcher club, state-of-the-art facilities, central location).
The team “Biology of phagocytes, infection and immunity” hosts 17 members. www.institutcochin.fr
Within this team, Anne Hosmalin's group develops projects on Dendritic cells against HIV : interferons alpha, lambda, cross-presentation
https://institutcochin.fr/projet-6-cellules-dendritiques-contre-vih-interferons-alpha-lambda-presentation-croisee.
The group is expert in studying interactions between human DC and HIV-infected cell (Hoeffel, Immunity, 2007 ; 10.1016/j.immuni.2007.07.02. Nascimbeni, Blood. 2009 ; 10.1182/blood-2008-07-170803. Iannetta, Front Immunol 2020, 10.3389/fimmu.2020.01658. Isnard, J. Immunol. 2021, 207: 15-22. doi: 10.4049/jimmunol.2000392. Isnard, en révision.) https://institutcochin.fr/en/project-6-dendritic-cells-against-hiv-alpha-and-lambda-interferons-cross-presentation

Compensation and benefits

Compensation

3 132,83€ gross per month depending on professional experience in equivalent positions

Annual leave and RTT

44 jours

Remote Working practice and compensation

Pratique et indemnisation du TT

Transport

Prise en charge à 75% du coût et forfait mobilité durable jusqu’à 300€

About the offer

About the CNRS

The CNRS is a major player in fundamental research on a global scale. The CNRS is the only French organization active in all scientific fields. Its unique position as a multi-specialist allows it to bring together different disciplines to address the most important challenges of the contemporary world, in connection with the actors of change.

CNRS

The research professions