Missions

Under the scientific supervision of Associate Professor Claire DUGAST-DARZACQ, and in close collaboration with a PhD student, the research engineer will actively contribute to the development of transgenic mouse embryonic stem cell (mESC) lines and their use in spinal organoid differentiation protocols. The engineer will provide technical and methodological support for these studies within the framework of the RIBES/NEDELEC research team's projects.

Activities

Transgenic Cell Line Creation and Management
-Participation in the generation of transgenic mESC lines.
-Establishment and monitoring of cell banking (freezing, thawing, maintenance).
Cell Differentiation and Organoid Production
-Development, adaptation, and optimization of differentiation protocols for mESC into spinal organoids.
-Morphological and phenotypic monitoring of cultures.
Molecular and Phenotypic Characterization
-Genotyping analyses (PCR, Sanger sequencing, or NGS).
-Immunohistochemistry and confocal imaging of organoids.
-Contribution to the analysis and interpretation of experimental data.
Scientific and Technical Support
-Support to the PhD student and contribution to collaborative work with the Associate Professor.
-Writing, updating, and sharing of experimental protocols.
-Participation in maintaining a laboratory environment that complies with safety and traceability standards.

Skills

Proficiency in stem cell culture (ideally mouse embryonic stem cells, mESC).
Knowledge of molecular biology techniques (PCR, cloning, sequencing).
Experience in immunohistochemistry and confocal microscopy.
Ability to work effectively in a team, with strong organizational skills and scientific rigor.

Work Context

This position is part of the research project developed by the RIBES/NEDELEC team, which brings together around fifteen researchers, students, postdoctoral fellows, and engineers. The team focuses on the generation and study of spinal cord development, notably through mouse and human organoid approaches.

Constraints and risks

-Work in a BSL-2 laboratory, with strict compliance with biosafety procedures.
-Exposure to noisy environments (centrifuges, hoods, heavy equipment).
-Occasional weekend presence required for monitoring cell cultures or long-term experiments.
-Handling of chemical reagents (solvents, fixatives) requiring adherence to good laboratory practices.

Additional Information

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