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Reference : UMR7592-ODIPOR-001
Workplace : PARIS 13
Date of publication : Friday, January 29, 2021
Type of Contract : FTC Scientist
Contract Period : 30 months
Expected date of employment : 1 June 2021
Proportion of work : Full time
Remuneration : from 2 728 € gross salary depending on experience
Desired level of education : Higher than 5-year university degree
Experience required : 1 to 4 years
A post-doctoral position is open to participate in a project investigating the molecular basis of juvenile ALS.
Amyotrophic lateral sclerosis (ALS) is the most common neurodegenerative disease affecting motor neurons. About 10% of ALS cases are inherited and due to dominant mutations in genes that very encode proteins involved in RNA metabolism. A juvenile form of this disease (ALS4) is caused by gain-of-function mutations in an RNA helicase involved in transcription termination and gene regulation. However, the connection between dysfunctions in these processes and spinal motor neuron degeneration remains unknown. To approach this question, we will use new methods to generate human motor neurons from induced pluripotent stem cells (Mouilleau, Vaslin et al, bioRxiv 2020.06.27.175646) on which we will apply biochemical, proteomic, genomic and cell biology approaches to elucidate the molecular pathway leading to motor neuron impairment in juvenile ALS. Our ultimate goal is to gain insight into the mechanisms linking RNA dysregulation to neurodegeneration.
This is a collaborative project funded by the FRM (Fondation pour la Recherche Medical) that will be carried out at the Institut Jacques Monod and the Institut du Fer à Moulin. The successful candidate will work under the supervision of O. Porrua (IJM) and S. Nedelec (IFM). He/she will integrate the two teams, and receive appropriate training from them.
More information about the host teams in https://equipelibri1.wixsite.com/librilab for O. Porrua and https://ifm-institute.org/equipe/nedelec/ for S. Nedelec.
The successful candidate will:
• Generate healthy and ALS4 motor neurons by differentiation of human induced pluripotent stem cells.
• Employ cell biology approaches to characterize the effect of ALS4 mutations on motor neuron physiology.
• Conduct proteomic and high-resolution genomic experiments to address the molecular phenotypes associated with ALS4 mutations.
• Participate in data bioinformatic analyses.
• Communicate the results of his/her project in meetings.
• Contribute to writing research articles.
• Highly motivated, well-organized and team-player.
• A PhD degree in Biochemistry, Cellular Biology, Molecular Biology or similar.
• Solid background in molecular biology and mammalian cell culture.
• Experience in genomics and some notions of bioinformatics.
• Expertise in stem cell biology and/or neurosciences would be an advantage.
• Capacity to speak and write in English.
• Applications from research engineers will be also considered and the level of responsibility in the project will be modulated accordingly.
The project will be developed in both the Institut Jacques Monod (IJM, https://www.ijm.fr/) and the Institut du Fer à Moulin (IFM, https://ifm-institute.org/).
The Institut Jacques Monod (IJM), located on the new campus of the Paris left bank urban project, is one of the main centers of fundamental research in biology in Paris and offers an international and multidisciplinary research environment. The IJM brings together thirty research groups working on cell and developmental biology, biophysics and genomics.
The Institut du Fer à Moulin, located in the center of Paris, is devoted to the study of the physiology and pathophysiology of the nervous system, with an important focus on the discovery of new therapeutic approaches.
The postdoctoral researcher will benefit from the stimulating scientific environments and state-of-the-art core facilities (cell and tissue imaging, proteomics, genomics, etc) of the host institutes.
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