Missions

The biological engineer's mission will be to select, adapt and implement methods to study the early development of marine embryos, including the production of recombinant proteins, mRNA, morpholinos, and eventually micro-injection and quantitative microscopy on living samples. He/she will work within the framework of scientific projects of the “Cellular Spatial Organization” team (www.minclab.fr).

The engineer recruited will be primarily responsible for a scientific aspect of the team, to develop new molecular tools for the study of embryonic development, but will also eventually have a mission to organize the team (lab manager). The initial contract will be for 18 months, but may be extended to 5 years.

Activities

- Purify recombinant proteins, messenger RNA or cytoplasmic extracts to isolate organelles.
- Validate these constructs using micro-injection adapted for marine embryos, for molecules (mRNA, morpholino, Crispr-CAS9, recombinant proteins),...
- Develop and adapt protocols, keep them up to date and archive them.
- Write reports and technical notes concerning the development of new methods that could benefit the institute and the university.
- Ensure the smooth running of equipment and stock management, particularly for molecular biology, biochemistry and micro-injection.
- Participate in cell biology/development and quantitative biology, involving large sample microscopy.
- Ensure compliance with health and safety regulations, as well as regulatory and administrative management of the animals and biological materials used.

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Skills

- In-depth skills in molecular biology and biochemistry, particularly in protein purification.
- General knowledge of cell biology and embryonic development biology.
- English language (scientific English) level B1 to B2, according to the Common European Framework of Reference for Languages.

Work Context

The “Cellular Spatial Organization” team focuses on embryonic development, tissue morphogenesis and cell division. The team's approaches are interdisciplinary, at the frontier between cell and developmental biology and theoretical and experimental physics. The team's main biological model is the sea urchin embryo, which offers numerous advantages for microscopy, biophysics and micro-injection. More information is available on the team's website: www.minclab.fr .

The engineer recruited will have a mainly scientific role and will work in collaboration with various team members (post-docs, students, etc.). He/she will also take part in team organization (stock management, equipment, etc.).

Constraints and risks

No particular risk