Informations générales
Intitulé de l'offre : Post-doc in genetics/genomics/evolution-M/W (H/F)
Référence : UMR7592-JEAFER-002
Nombre de Postes : 1
Lieu de travail : PARIS 13
Date de publication : vendredi 10 novembre 2023
Type de contrat : CDD Scientifique
Durée du contrat : 24 mois
Date d'embauche prévue : 1 avril 2024
Quotité de travail : Temps complet
Rémunération : From 2 932 € to 4 204 € gross per month depending on experience
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : 1 à 4 années
Section(s) CN : Interactions, particles, nuclei, from laboratory to cosmos
Missions
Managing a research project
Objective: creating a Vibrio cholerae strain adapted to DnaC, tracing the modifications and their succession during adaptation to DnaC to understand the mechanism underlying bidirectional replication initiation.
Activités
- Setting up the evolutionary screen assay
- Construction of the screen strain
- Construction of the plasmids of complementation
- Building a viable screen strain
- Steady state culture of cells and selection of cells with improved fitness
- Whole genome sequence of adapted cells
- Bioinformatics to identify the adaptive mutations
Compétences
- Molecular biology (needed)
- Genetics (needed)
- Bioinformatics (would be a plus – formation possible)
- Evolutionary biology (would be a plus)
- Cytology (would be a plus)
Contexte de travail
The host team
The team comprises 3 permanent positions and 3 PhD students, working on the initiation of replication in vertebrates (human, chicken) and bacteria (Vibrio cholerae, Escherichia coli). The project will be supervised by Jean-Luc Ferat who is a professor at University Paris Cité.
The IJM
The Institut Jacques Monod is a joint research unit of CNRS and Université Paris Cité. The Institute is ideally located on the left bank of Paris. The institute is a fundamental research center with some thirty groups covering the fields of genome and chromosome dynamics, cell dynamics and signaling, development and evolution with a rich scientific life.
Contraintes et risques
none