Missions

Managing a research project
Objective: creating a Vibrio cholerae strain adapted to DnaC, tracing the modifications and their succession during adaptation to DnaC to understand the mechanism underlying bidirectional replication initiation.

Activités

- Setting up the evolutionary screen assay
- Construction of the screen strain
- Construction of the plasmids of complementation
- Building a viable screen strain
- Steady state culture of cells and selection of cells with improved fitness
- Whole genome sequence of adapted cells
- Bioinformatics to identify the adaptive mutations

Compétences

- Molecular biology (needed)
- Genetics (needed)
- Bioinformatics (would be a plus – formation possible)
- Evolutionary biology (would be a plus)
- Cytology (would be a plus)

Contexte de travail

The host team
The team comprises 3 permanent positions and 3 PhD students, working on the initiation of replication in vertebrates (human, chicken) and bacteria (Vibrio cholerae, Escherichia coli). The project will be supervised by Jean-Luc Ferat who is a professor at University Paris Cité.

The IJM
The Institut Jacques Monod is a joint research unit of CNRS and Université Paris Cité. The Institute is ideally located on the left bank of Paris. The institute is a fundamental research center with some thirty groups covering the fields of genome and chromosome dynamics, cell dynamics and signaling, development and evolution with a rich scientific life.

Contraintes et risques

none