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Reference : UMR7587-OLITHO-001
Workplace : PARIS 05
Date of publication : Monday, November 18, 2019
Type of Contract : FTC Scientist
Contract Period : 18 months
Expected date of employment : 27 January 2020
Proportion of work : Full time
Remuneration : Between 2700 and 3200 euros of gross salary per month depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
Retinal diseases cause varying degrees of vision loss up to complete blindness. Stem cell therapy can offer the possibility of recuperating vision. The Vision Institute uses human induced pluripotent stem (iPS) cell technology to generate different retinal cell types for rescue or replacement strategies in patients. 3D retinal organoids contain all major retinal cell types and a distinct layering close to in vivo morphology, and therefore hold great promise for transplantation, as well as for disease modeling and drug development.
The project aims to develop a non-invasive 3D live imaging microscope based on dynamic full-field optical coherence tomography (FFOCT), a technique invented at the Langevin Institute. It will fulfil an unmet need for in vitro screening of 3D retinal organoids and 2D cell cultures developed at the Vision Institute for eventual graft and new therapies evaluation.
The candidate will design this microscope optimized for the longitudinal study of live organoids. He will build the microscope at the Vision Institute and will start performing the longitudinal evaluation of the organoids. The candidate will participate in the precise characterization of the observed signal, and to the automatic analysis of the organoid main features (cell density, cell types, activity, etc..)
A second post-doc project of 18 months as well will follow this one and will use the microscope built during this first period to perform longitudinal studies on healthy and pathological retinal organoids, as well as pharmacological drugs evaluation. Therefore, the current project could, if desired, be extended to a 3 years project combining the instrumentation and biological parts.
Candidates with a PhD in physics, optics, electrical engineering, neuroscience or fundamental biology (with some experience in optical imaging) or any related field are encouraged to apply. As this project is tightly linked to its applications in biology, the candidates are expected to have a strong interest in neuroscience and biology, and /or clinical applications.
Programming skills are essential (Matlab or Python). As they will be evolving in an international and collaborative environment, the candidates must be fluent in English, and show excellent communications and organizational capabilities.
This project is at the core of a new ANR project called OREO that brings together researchers from the Langevin Institute, the Vision Institute, and the 15-20 national ophthalmology hospital. The final aim of OREO is to provide a new instrument that will evaluate the structure and function of retinal organoids from diseased patients and how they respond to potential new treatments of such diseases.
The project will be carried out at the Langevin Institute in the center of Paris, and some experiments will be conducted in the Vision Institute 15 minutes away. The Langevin Institute gathers around 100 people around the physics of waves (optics, acoustics, electromagnetics, water waves, etc…) and their interaction with different media, including biological samples. The Vision Institute and 15-20 national ophthalmology hospital also gathers many scientists, mostly biologists or clinicians, around the functions and dysfunctions of the eye.
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