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Reference : UMR7361-KARANS-003
Workplace : MULHOUSE
Date of publication : Wednesday, November 18, 2020
Type of Contract : FTC Technical / Administrative
Contract Period : 36 months
Expected date of employment : 4 January 2021
Proportion of work : Full time
Remuneration : between 2 675€ and 3 805 € gross per month according to experience
Desired level of education : PhD
Experience required : 1 to 4 years
The research engineer will be in charge of the functionalization of surfaces with amino-rich nanolayers by various techniques (plasma polymer, self-assembled monolayers) and their physico-chemical characterization. He/she will proceed to the qualitative and quantitative analysis of serum proteins adsorbed on these various amino-rich surfaces (experience in liquid chromatography techniques is strongly recommended). Afterwards, he/she will participate to the preparation and analysis of samples for in vitro cellular experiments. He/she should be highly motivated, dynamic, resourceful and willing to integrate a multidisciplinary team and international network, open to new ideas and concepts, and have a strong taste for interdisciplinary sciences at the interface of chemistry, biology, and physics.
- functionalization of surfaces with amino-rich nanolayers by various techniques (plasma polymer, self-assembled monolayers)
- characterization of surfaces with amino-rich nanolayers by various techniques (XPS, AFM, wettability)
- quantitative analysis of serum proteins adsorbed on the amino-rich nanolayers (gel electrophoresis and LC/MS analysis of proteins after desorption, quantification in supernatants by SE-HPLC)
- conformation analysis of adsorbed proteins (ATR-FTIR)
- characterization of grafted amino-rich polymer nano-layers prepared by German partners
- preparation and analysis of samples for in vitro cellular experiments
The research engineer will need a solid formation in physical-chemical analysis and surface functionalization, and a good knowledge in protein adsorption and grafting at biomaterial surfaces.
An experience in liquid chromatography techniques is strongly recommended.
This three-year research engineer position supported by the International France-Germany ANR-DFG grant “Amino-coat” is available at the Institute of Materials Science of Mulhouse (IS2M) UMR CNRS 7361, France (http://www.is2m.uha.fr/) under the direction of Dr Arnaud Ponche from the “Biomaterials-Biointerfaces” research group and Dr Florence Bally-Le Gall from the “Functional polymers engineering” research group. The successful candidate will also work in collaboration with the group of Pr Matthias Schnabelrauch at INNOVENT, Jena, Germany (https://www.innovent-jena.de/en/).
Project Title: Aminocoatings for improving implants' tissue integration: understanding underlying biological mechanisms
Cells can respond to extracellular cues of very different natures that can be, for instance, biochemical factors or topography. It was shown that when cells grow on groove patterns, they align along the groove direction. This response can be inhibited by the deposition of a nanometric poly(allylamine) plasma polymer coating. Understanding how the cells integrate these signals is essential to develop efficient tissue engineering strategies.
The Amino-coat project aims at elucidating the mechanism by which chemical signals can mask topographical cues during the cell response to geometrically grooved titanium substrates. The project associates biologists specialized in cell/materials interactions from Dr K. Anselme's group at IS2M and from Pr J.B. Nebe's group (German coordinator of the project) at the University of Rostock, Germany (https://zellbiologie.med.uni-rostock.de/arbeitsgruppen) and physicists, specialists in cell modelling (Dr J.L. Milan group) from the Aix-Marseille University, France (https://ism.univ-amu.fr/en/giboc).
The Institute of Materials Science of Mulhouse gathers about 180 people including 60 permanent researchers with skills in chemistry, physics and biology. Mulhouse is in the typical Alsace region in eastern France at the border with Germany (Freiburg im Breisgau) and Switzerland (Basel). It has a direct access to Paris (less than 3 hours by TGV) and to an international airport (Euroairport, 20km).
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