Missions

The candidate will be recruited for the France 2030 RNAvac project (2023-2027), which aims to optimize mRNA vaccine formulations using preclinical models. Responsibilities include:
- Developing and implementing experimental protocols in biology and animal experimentation.
- Conducting in vitro and in vivo experiments, including models of intestinal inflammation and Citrobacter rodentium infection.
- Contributing to the publication of results in international journals.
- Participating in the supervision of interns and PhD students within the team.

Activités

Main Activities:
- Develop a colorectal cancer model through C. rodentium infection and chemical agents.
- Set up and conduct experimental protocols on small animals within the research project.
- Perform experiments in cellular and molecular biology (cell and bacterial culture, PCR, RT-PCR, flow cytometry).
- Characterize tissue samples using histology, immunohistochemistry, and immunofluorescence.
- Analyze, interpret, and present results.
- Maintain a laboratory notebook and project tracking sheet (ISO 9001:2015 Quality Standard).
- Conduct scientific and technological monitoring related to the research project.
- Manage biological reagent and consumable stocks necessary for experiments.
- Participate in collective tasks (media preparation, equipment maintenance, etc.).

Associated Activities:
- Participate in team meetings and seminars.
- Present results at conferences.
- Write scientific articles.
- Contribute to the supervision of PhD students and interns.

Compétences

Knowledge:
- PhD in Immunology
- Advanced technical skills in molecular and cellular biology
- In-depth knowledge of animal experimentation
- Expertise in multi-parameter flow cytometry, microscopy, and imaging techniques
- Strong background in scientific data analysis

Skills:
- Ability to develop new preclinical study models
- Writing project authorization requests and using the APAFiS platform
- Proficiency in flow cytometry analysis software

Soft Skills:
- Rigor and organization in conducting experiments
- Teamwork skills
- Fluent communication in English
- Autonomy in project management

Contexte de travail

Laboratory of Immuno-Neuro Modulation (INEM, formerly the Laboratory of Experimental and Molecular Immunology and Neurogenetics), research Unit UMR7355, located on the CNRS campus in Orléans.The unit consists of two research teams (20 researchers and faculty members, 10 technical staff, and 10 PhD students and interns) and is equipped with state-of-the-art facilities, including:
- Cell culture (Biosafety Level 1 and 2)
- Histology (tissue processing automaton, microtomes, cryostats)
- Pulmonary function analysis (invasive and non-invasive plethysmographs)
- Animal experimentation ( A1 and A2 animal facilities)
The recruited candidate will work under the supervision of Prof. Dieudonnée TOGBE.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

The risks are typical of an experimental biology laboratory:
- Working in a Level 2 confined environment
- Handling biological materials (human cells and bacteria)
- Handling chemical substances (reagents required for experiments)
- Risks associated with laboratory equipment (centrifuges, lasers)
- Strict compliance with laboratory hygiene and safety regulations
- Mandatory training in animal experimentation