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Reference : UMR7327-MARROU0-011
Workplace : ORLEANS
Date of publication : Wednesday, September 16, 2020
Type of Contract : FTC Scientist
Contract Period : 9 months
Expected date of employment : 1 November 2020
Proportion of work : Full time
Remuneration : Between 2648 € and 3768 € gross monthly depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
At CNRS-ISTO (Earth Sciences Institute of Orléans, the recruited person will be part of the Continental Biogeosystems team of ISTO to work within the framework of the BIENSUR project (Microbial and plant biodiversities IN URBAN soils within the framework of the "Zero phyto Plan") co-funded by the Centre-Val-de-Loire Region. The main objective of this project is to assess the effects of stopping the use of phytosanitary products and alternative techniques for managing natural spaces in the city without these products, both on the floristic and microbial diversity of urban soils and the impact on the population (citizens, gardeners, green space agents). In this context, the person will coordinate the soil sampling campaigns and all the measurements of the microbiological and physico-chemical parameters: enzymatic measurement approaches, diversity and microbial activity, PLFA measurements and biogeochemical determinations TOC, C, H , N, S, C and N microbial organic…) in connection with the various partners and in particular with the Geomicrobiology and Environmental Monitoring unit of BRGM, partner of the project.
As part of the BIENSUR project, the recruited person will be responsible for coordinating ecological samples and diagnostics of urban soils (public green spaces and private gardens, technosols (buildings, roads, etc.) selected in concertation with the partners. They will also contribute to the identification and definition of the management gradients to target (use of past phytosanitary products vs never treated, intensity of management with the various alternative techniques). The person will be in charge of managing the determinations of the physico-chemical characteristics of the soils (pH, texture, density, organic matter, nutrients, etc.), genetic diversity and the functional diversity of soil microbial communities (BRGM, ISTO).
These microbial characterizations of soils will include measures of abundance, diversity (T-RFLP) and functions (linked to the cycle of carbon, nitrogen, sulfur and phosphorus). In addition, a biochemical characterization of the communities will be carried out with the measurement of PLFAs: membrane phospholipids (information on a set of microbial characters, i.e. biomass, physiology, taxonomic and functional identity, and composition of the community).
• Validated skills in environmental soil microbiology, particularly in bio-indication approaches;
• Ability to carry out multidisciplinary field studies and to design and use experimental devices;
• Capacity for statistical analysis of ecological data (knowledge of R Stats is a plus);
• Skills validated for aerobic and anaerobic bacterial cultures;
• Knowledge of imaging techniques (electron microscopy, optical microscopy especially in fluorescence ...) and molecular microbiology techniques;
• Knowledge of PLFA analysis techniques;
• Ability to work independently, and to work in a team
• Ability to communicate and report
• Fluency in English (read, written, spoken), current French;
It is expected that the data obtained will enable the publication of a scientific paper. You shall also contribute to the project of restitution of the major results of the project to the general public.
The position is based in Orléans (France) at ISTO with round trips to the neighboring BRGM (Orléans) and trips to the study areas (Blois).
Constraints and risks
Short trips to France and if necessary abroad are to be expected.
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