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Reference : UMR7313-MICLAF-008
Workplace : MARSEILLE 13
Date of publication : Friday, November 13, 2020
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 February 2021
Proportion of work : Full time
Remuneration : Between 2728,26€ and 3145,68€ gross/month
Desired level of education : PhD
Experience required : 1 to 4 years
The researcher will be hired for 12 months, with the possibility of a 6-months extension, in the framework of the ANR-Funtastic project (2017-2022) funded to explore the diversity of fungal Copper-Radical Oxidases (CROs) and investigate their molecular mechanism through a structure-activity study. The overall goal of the project is to upgrade biomass components, in particular complex carbohydrates and alcohols through a biocatalytic route.
The missions of the researcher will be to heterologously produce, functionally and structurally characterize novel CROs from the AA5-2 subfamily (according to the CAZy database classification) and their redox partners.
He/she will be i) involved in the heterologous production via the Pichia pastoris expression system of novel CROs, ii) their functional and structural characterizations, and iii) the study of the CROs/redox partners interactions.
Applicant should have good knowledge in biochemistry, molecular biology and structural biology and expertise in :
- heterologous expression, ideally in P. pastoris and protein purification using FPLC system
- biochemical and enzymatic characterization
- protein/protein interaction
Knowledge in protein crystallography will be valuable for the project.
Since 2014, a close collaboration between Dr. M. Lafond (CNRS-ISM2-UMR7313), Dr. J-G. Berrin (INRAE-BBF-UMR1163), and Pr. H. Brumer (MSL-UBC, Canada) has been developed through the study of fungal CROs from subfamily AA5_2.
The researcher will carry out his research mainly at the BBF-INRAE laboratory (Marseille) but also at ISM2-BiosCiences (Marseille) under the co-supervision of Drs. M. Lafond and J-G. Berrin. Protein crystallization will be carried out at AFMB-CNRS/INRAE in collaboration with Dr. G. Sulzenbacher.
Constraints and risks
No particular constraints and risks.
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