Reference : UMR7284-VALVOU-002
Nombre de Postes : 1
Workplace : NICE
Date of publication : Monday, January 30, 2023
Type of Contract : FTC Scientist
Contract Period : 12 months
Expected date of employment : 1 March 2023
Proportion of work : Full time
Remuneration : gross salary from 2833,40 € to 4353,15 € depending on experience
Desired level of education : PhD
Experience required : 1 to 4 years
Post-doctoral researcher in charge of developing and conducting experimental protocols in the framework of a research project on immunotherapy and tumor microenvironment. The position is open for a period of 2 to 3 years. First contract of one year with the possibility of an extension for the remaining period. Salary depends on the candidate's experience, in accordance with CNRS guidelines.
Our previous research highlighted that purinergic immune checkpoint (PC: P2RX7, CD39, CD73 and A2AR) and extracellular ATP are the basis for new therapies to treat lung cancer. The objectives of the project are to 1/ characterize the expression and function of PCs in non-small cell lung adenocarcinoma, 2/ optimize the 3D culture of tumor tissues from patients, and 3/ develop new therapies by directly targeting PCs that will be tested on murine lung cancer models and patient-derived organoids.
-Spatial profiling of purinergic actors expressed in lung tumors (transcriptomics and proteomics)
-3D culture of human tissues (lung cancer) and treatments.
-Treatments (chemical and biological molecules) in mice.
-Sharing of raw data
-Formatting of the results and the statistical analysis.
-Follow-up of the literature, improvement/adaptation of techniques and analyses
-Participate in the supervision of students (master or doctoral student)
-PhD in immunology, cell biology, physiology, or related disciplines
-In-depth knowledge of immunology and intracellular signaling pathways
-Experience in 3D culture
-Experience in animal experimentation (level 1 or 2)
-Conducting flow cytometry analysis
-Great rigor, precision and organization in the realization of experiments
-Ability to understand and design new techniques/devices
-Ability to work in a team
-Ability to communicate in spoken and written English
-Ability to travel for training and collaboration
The group led by Valérie Vouret is part of Prof P. Hofman's team at IRCAN (ircan.org) and has been working in the field of purinergic signaling and cancer for several years. The group is funded by Europe (Transcan) and by several national projects (ARC, Cancéropole, Inca). The Institute for Research on Cancer and Aging of Nice (IRCAN) is a joint research unit (UMR 7284) of CNRS, INSERM and the University of Côte d'Azur located east of Nice, near the Pasteur Hospital. The 15 IRCAN teams benefit from high-level technical platforms (Biobank, 3D culture, cytometry, imaging, genomics, bioinformatics).
Constraints and risks
Standard risks associated with working with animals and the use of chemical and biological products.
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